RegulonDB RegulonDB 10.8: Operon Form
   

lexA-dinF operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: lexA-dinF
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: lexA-dinF
Synonym(s): OP00046, lexA
Gene(s): lexA, dinF   Genome Browser M3D Gene expression COLOMBOS
Evidence: [PM] Polar mutation
Reference(s): [1] Krueger JH., et al., 1983
Promoter
Name: lexAp
+1: 4257087
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 28
Sequence: ctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcAtaactgtatatacacccagg
                          -35                    -10        +1                   
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Miki T., et al., 1981
[3] Salgado H, et al., 2012
TF binding sites (TFBSs)
[LexA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor lexAp 4257027 4257046 -50.5 ttcgataaatCTCTGGTTTATTGTGCAGTTtatggttcca nd [APIORCISFBSCS] [4], [5]
proximal LexA repressor lexAp 4257068 4257087 -9.0 aatcgcctttTGCTGTATATACTCACAGCAtaactgtata nd [APIORCISFBSCS], [BPP] [5], [6]
proximal LexA repressor lexAp 4257089 4257108 13.0 ctcacagcatAACTGTATATACACCCAGGGggcggaatga nd [APIORCISFBSCS], [BPP] [5], [6]


Evidence    

 [CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)

 [CV(TIM)] cross validation(TIM)

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Krueger JH., Elledge SJ., Walker GC., 1983, Isolation and characterization of Tn5 insertion mutations in the lexA gene of Escherichia coli., J Bacteriol 153(3):1368-78

 [2] Miki T., Ebina Y., Kishi F., Nakazawa A., 1981, Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region., Nucleic Acids Res 9(3):529-43

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Lewis LK., Harlow GR., Gregg-Jolly LA., Mount DW., 1994, Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli., J Mol Biol 241(4):507-23

 [5] Little JW., Mount DW., Yanisch-Perron CR., 1981, Purified lexA protein is a repressor of the recA and lexA genes., Proc Natl Acad Sci U S A 78(7):4199-203

 [6] Brent R., Ptashne M., 1981, Mechanism of action of the lexA gene product., Proc Natl Acad Sci U S A 78(7):4204-8

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