RegulonDB RegulonDB 10.6.3: Operon Form
   

malEFG operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: malEFG
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: malEFG
Synonym(s): OP00047, malE
Gene(s): malG, malF, malE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ITCR] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Francoz E., et al., 1988
Promoter
Name: malEp
+1: 4246464
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 45
Sequence: cggtggggcgtaggggcaaggaggatggaaagaggttgccgtataaagaaactagagtccGtttaggtgttttcacgagca
                          -10                        -35    +1                   
Evidence: [GEA]
[IMP]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Bedouelle H. 1983
[3] Richet E. 1996
[4] Salgado H, et al., 2012
[5] Typas A., et al., 2007
Terminator(s)
Type: rho-independent
Sequence: agaaaaaaagGCGCTCCGTGGAGCGCCgaataacagt
Reference(s): [1] Francoz E., et al., 1988
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator malEp 4246530 4246551 -76.5 ttaccgccaaTTCTGTAACAGAGATCACACAAagcgacggtg nd [BPP], [HIBSCS], [SM] [8], [10], [11], [13]
remote CRP-cAMP activator malEp 4246559 4246580 -105.5 cgtggcgattTTATGTGCGCATCTCCACATTAccgccaattc nd [AIBSCS], [BPP], [HIBSCS], [SM] [7], [8], [10], [11]
remote CRP-cAMP activator malEp 4246593 4246614 -139.5 atgtaaggaaTTTCGTGATGTTGCTTGCAAAAatcgtggcga nd [BPP], [HIBSCS], [SM] [8], [10], [11]
remote CRP-cAMP activator malEp 4246625 4246646 -171.5 agaacacggcTTCTGTGAACTAAACCGAGGTCatgtaaggaa nd [AIBSCS], [BPP], [SM] [7], [8], [10]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CreB-Phosphorylated repressor malEp 4246438 4246456 18.5 ccgtttaggtGTTTTCACGAGCACTTCACcaacaaggac nd [AIBSCS], [GEA] [14]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis activator malEp 4246404 4246423 52.0 aaggaccataGATTATGAAAATAAAAACAGgtgcacgcat nd [AIBSCS], [GEA] [12]
remote Fis activator malEp 4246571 4246590 -116.0 ttgcaaaaatCGTGGCGATTTTATGTGCGCatctccacat nd [AIBSCS], [GEA] [12]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MalT-maltotriose-ATP activator malEp 4246501 4246510 -41.5 ggggcgtaggGGCAAGGAGGatggaaagag nd [BPP], [HIBSCS], [SM] [3], [6], [7], [8], [9]
proximal MalT-maltotriose-ATP activator malEp 4246511 4246520 -51.5 aagcgacggtGGGGCGTAGGggcaaggagg nd [AIBSCS], [BPP], [HIBSCS], [SM] [3], [6], [7], [8], [9]
remote MalT-maltotriose-ATP activator malEp 4246657 4246666 -197.5 ggggaggaggCGGGAGGATGagaacacggc nd [AIBSCS], [BPP], [SM] [3], [6], [7], [8], [9]
remote MalT-maltotriose-ATP activator malEp 4246667 4246676 -207.5 cttttttatgGGGGAGGAGGcgggaggatg nd [BPP], [HIBSCS], [SM] [3], [6], [7], [8], [9]
remote MalT-maltotriose-ATP activator malEp 4246691 4246700 -231.5 tggcttaaatCCTCCACCCCctggcttttt nd [BPP], [HIBSCS], [SM] [3], [6], [7], [8], [9]




Reference(s)    

 [1] Francoz E., Dassa E., 1988, 3' end of the malEFG operon in E.coli: localization of the transcription termination site., Nucleic Acids Res 16(9):4097-109

 [2] Bedouelle H., 1983, Mutations in the promoter regions of the malEFG and malK-lamB operons of Escherichia coli K12., J Mol Biol 170(4):861-82

 [3] Richet E., 1996, On the role of the multiple regulatory elements involved in the activation of the Escherichia coli malEp promoter., J Mol Biol 264(5):852-62

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Typas A., Barembruch C., Possling A., Hengge R., 2007, Stationary phase reorganisation of the Escherichia coli transcription machinery by Crl protein, a fine-tuner of sigma(s) activity and levels., EMBO J 26(6):1569-78

 [6] Boos W., Shuman H., 1998, Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation., Microbiol Mol Biol Rev 62(1):204-29

 [7] Raibaud O., 1989, Nucleoprotein structures at positively regulated bacterial promoters: homology with replication origins and some hypotheses on the quaternary structure of the activator proteins in these complexes., Mol Microbiol 3(3):455-8

 [8] Raibaud O., Vidal-Ingigliardi D., Richet E., 1989, A complex nucleoprotein structure involved in activation of transcription of two divergent Escherichia coli promoters., J Mol Biol 205(3):471-85

 [9] Richet E., Raibaud O., 1991, Supercoiling is essential for the formation and stability of the initiation complex at the divergent malEp and malKp promoters., J Mol Biol 218(3):529-42

 [10] Vidal-Ingigliardi D., Raibaud O., 1991, Three adjacent binding sites for cAMP receptor protein are involved in the activation of the divergent malEp-malKp promoters., Proc Natl Acad Sci U S A 88(1):229-33

 [11] Richet E., 2000, Synergistic transcription activation: a dual role for CRP in the activation of an Escherichia coli promoter depending on MalT and CRP., EMBO J 19(19):5222-32

 [12] Bradley MD., Beach MB., de Koning AP., Pratt TS., Osuna R., 2007, Effects of Fis on Escherichia coli gene expression during different growth stages., Microbiology 153(Pt 9):2922-40

 [13] Merkel TJ., Nelson DM., Brauer CL., Kadner RJ., 1992, Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene., J Bacteriol 174(9):2763-70

 [14] Avison MB., Horton RE., Walsh TR., Bennett PM., 2001, Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media., J Biol Chem 276(29):26955-61


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