RegulonDB RegulonDB 10.10: Operon Form
   

hflD-purB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hflD-purB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: hflD-purB
Synonym(s): OP00075, purB, ycfC-purB
Gene(s): purB, hflD   Genome Browser M3D Gene expression COLOMBOS
Note(s): The purB promoter was removed from this transcription unit because new evidence showed that it does not exist Green SM,1996
Evidence: [BTEI] Boundaries of transcription experimentally identified
[PM] Polar mutation
Reference(s): [1] Green SM., et al., 1996
[2] Meng LM., et al., 1990
Promoter
Name: hflDp
+1: 1192686
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 55
Sequence: gccagtctgccgtcttctataacggtgaagtgtgcctcggtggcggtattattgagcagcGtctgccgctgccggtctgat
                         -35                   -10          +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Green SM., et al., 1996
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[PurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote PurR-hypoxanthine repressor hflDp 1191787 1191802 892.5 tttgctgccgACGCAATCGGTTACCTtgatgcaatc nd [GEA], [AIBSCS], [APIORCISFBSCS], [BPP] [4], [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Green SM., Malik T., Giles IG., Drabble WT., 1996, The purB gene of Escherichia coli K-12 is located in an operon., Microbiology 142 ( Pt 11):3219-30

 [2] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [5] He B., Smith JM., Zalkin H., 1992, Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR., J Bacteriol 174(1):130-6

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