RegulonDB RegulonDB 10.8: Operon Form
   

purC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: purC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: purC
Synonym(s): OP00076
Gene(s): purC   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on DNA microarray analysis, the mechanism of bacterial inactivation by carvacrol and citral was studied 28644990. Treatment by both compounds caused membrane damage and activated metabolism through the production of nucleotides required for DNA and RNA synthesis and metabolic processes 28644990. A total of 76 and 156 genes demonstrated significant transcriptional differences by carvacrol and citral, respectively. Genes upregulated by carvacrol treatment included the multidrug efflux pump genes acrA and mdtM, genes related to the phage shock response, pspA, pspB, pspC, pspD, pspF, and pspG, and genes whose products are important for biosynthesis of arginine (argC, argG, artJ) and purine nucleotides (purC, purM). Genes upregulated by citral treatment included purH, pyrB, and pyrI. On the other hand, mutations in several differentially expressed genes confirmed the roles of ygaV, yjbO, pspC, sdhA, yejG, and ygaV in mechanisms of inactivation by carvacrol and citral 28644990.
Reference(s): [1] Meng LM., et al., 1990
Promoter
Name: purCp
+1: 2597781
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 163
Sequence: ttagcaagtaaaaatacagggctggaatcatccggcccttttttctgatatgatacgcaaAcgtgtgcgtctgcaggaaaa
                      -35                        -10        +1                   
Evidence: [TIM]
Reference(s): [2] He B., et al., 1990
[3] Tiedeman AA., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor purCp 2597772 2597787 2.5 ctgatatgatACGCAAACGTGTGCGTctgcaggaaa nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [4]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -15.5 2597796 2597824 gtaaaaatacAGGGCTGGAATCATCCGGCCCTTTTTTCtgatatgata
  anti-terminator -9.2 2597815 2597854 ggtagctgtcTTCCAGGCTGCGTTTAGCAAGTAAAAATACAGGGCTGGAatcatccggc
  anti-anti-terminator -10.4 2597841 2597872 gagtcagaacGACGCGCTGGTAGCTGTCTTCCAGGCTGCGTttagcaagta
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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