|Gene(s):||purD, purH Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||The site for the RbsR transcriptional regulator in the purHD operon was identified based on genomic Selex analysis Shimada T,2013
The deletion of the Fis DNA binding sites located upstream of the purHp promoter enhances the inhibition of purHp via transcription-coupled DNA supercoiling (TCDS) in the early log phase. Fis appears to block supercoiling diffusion close to purHp 31639222.
Based on DNA microarray analysis, the mechanism of bacterial inactivation by carvacrol and citral was studied 28644990. Treatment by both compounds caused membrane damage and activated metabolism through the production of nucleotides required for DNA and RNA synthesis and metabolic processes 28644990. A total of 76 and 156 genes demonstrated significant transcriptional differences by carvacrol and citral, respectively. Genes upregulated by carvacrol treatment included the multidrug efflux pump genes acrA and mdtM, genes related to the phage shock response, pspA, pspB, pspC, pspD, pspF, and pspG, and genes whose products are important for biosynthesis of arginine (argC, argG, artJ) and purine nucleotides (purC, purM). Genes upregulated by citral treatment included purH, pyrB, and pyrI. On the other hand, mutations in several differentially expressed genes confirmed the roles of ygaV, yjbO, pspC, sdhA, yejG, and ygaV in mechanisms of inactivation by carvacrol and citral 28644990.
|Reference(s):|| Aiba A., et al., 1989|
|Sigma Factor:||Sigma70 Sigmulon|
|Distance from start of the gene:||95|
-35 -10 +1
 Aiba A., et al., 1989
 He B., et al., 1990
 Huerta AM., et al., 2003
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|proximal||PurR-hypoxanthine||repressor||purHp||4207640||4207655||-20.5||tcgcgagcgtTGCGCAAACGTTTTCGTtacaatgcgg||nd||[AIBSCS], [APIORCISFBSCS], [BPP], [GEA]||, |
|Transcription factor||Function||Promoter||Binding Sites||Evidence (Confirmed, Strong, Weak)||Reference(s)|
1This regulatory interaction was identified by means of PhoPQ-related microarray experiments, and it was investigated to determine the presence of its regulatory motif in the promoter region Monsieurs P,2005.1This regulatory interaction was identified by means of PhoPQ-related microarray experiments, and it was investigated to determine the presence of its regulatory motif in the promoter region Monsieurs P,2005.
|RNA cis-regulatory element|
|Regulation, transcriptional elongation|
|Structure type||Energy||LeftPos||RightPos||Sequence (RNA-strand)|
|Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"|
 Monsieurs P., De Keersmaecker S., Navarre WW., Bader MW., De Smet F., McClelland M., Fang FC., De Moor B., Vanderleyden J., Marchal K., 2005, Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium., J Mol Evol 60(4):462-74