RegulonDB RegulonDB 10.10: Operon Form
   

purR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: purR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: purR
Synonym(s): OP00081
Gene(s): purR   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Meng LM., et al., 1990
Promoter
Name: purRp
+1: 1737689
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 155
Sequence: ataatgataagtgtttttaccacttccccttttcgtcaagatcggccaaaattccacgctTacactatttgcgtactggcc
                        -35                    -10          +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [2] He B., et al., 1990
[3] Huerta AM., et al., 2003
[4] Rolfes RJ., et al., 1988
TF binding sites (TFBSs)
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor purRp 1737631 1737649 -49.0 cgtcatttatAATGATAAGTGTTTTTACCacttcccctt nd [AIBSCS] [6]
[PurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote PurR-hypoxanthine repressor purRp 1737784 1737799 103.5 aggtgtgtaaAGGCAAACGTTTACCTtgcgattttg nd [GEA], [APIORCISFBSCS], [BCE], [BPP], [SM] [1], [7], [8]
remote PurR-hypoxanthine repressor purRp 1737872 1737887 191.5 gtagcgaaacGAGCAAACGTTTCCACtacaactgtg nd [GEA], [APIORCISFBSCS], [BCE], [BPP], [SM] [1], [8]
sRNA Interaction TU
sRNA TU Regulated Function Binding Sites Regulatory Mechanism Evidence (Confirmed, Strong, Weak) Reference(s)
PosLeft PosRight Target sequence (mRNA)
small regulatory RNA SgrS purR repressor 1737892 1737910 ACUGUGUCACACGUGAUCA TRANSLATION-BLOCKING [IMP], [SM] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts

 [BPP] Binding of purified proteins

 [SM] Site mutation

 [IMP] Inferred from mutant phenotype


Reference(s)    

 [1] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

 [2] He B., Shiau A., Choi KY., Zalkin H., Smith JM., 1990, Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction., J Bacteriol 172(8):4555-62

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Rolfes RJ., Zalkin H., 1988, Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator., J Biol Chem 263(36):19653-61

 [5] Bobrovskyy M., Vanderpool CK., 2016, Diverse mechanisms of post-transcriptional repression by the small RNA regulator of glucose-phosphate stress., Mol Microbiol 99(2):254-73

 [6] Stojiljkovic I., Baumler AJ., Hantke K., 1994, Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay., J Mol Biol 236(2):531-45

 [7] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [8] Rolfes RJ., Zalkin H., 1990, Autoregulation of Escherichia coli purR requires two control sites downstream of the promoter., J Bacteriol 172(10):5758-66

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