RegulonDB RegulonDB 10.8: Operon Form
   

recAX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: recAX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: recAX
Gene(s): recX, recA   Genome Browser M3D Gene expression COLOMBOS
Note(s): recA-recX cotranscription results from partial readthrough at the recA terminator.
Evidence: [ITC] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Pages V., et al., 2003
[2] Stohl EA., et al., 2003
Promoter
Name: recAp
+1: 2823819
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 50
Sequence: cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc
                            -35                   -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Pages V., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor recAp 2823829 2823848 -20.0 aaacacttgaTACTGTATGAGCATACAGTAtaattgcttc nd [BPP] [3], [4]


Transcription unit          
Name: recA
Synonym(s): OP00086
Gene(s): recA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on the bacteriostatic antibiotic-induced persister model, 37 and 9 genes were found to produce defects in rifampin- and tetracycline-induced persister formation, respectively. Only six mutants were found to overlap in both treatment-induced persister screens: recA, recC, ruvA, uvrD, fis, and acrB. Four of these mutants (recA, recC, ruvA, and uvrD) mapped to the DNA repair pathway, one mutant mapped to a global transcriptional regulator (fis), and one to a gene for an efflux protein (acrB) 29559967 Other genes that mapped to transporters, membrane biogenesis, LPS biosynthesis, flagellum biosynthesis, metabolism (folate and energy), and translation were more specific to rifampin-induced persisters 29559967
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [5] Clark AJ., et al., 1994
[6] Fernandez De Henestrosa AR., et al., 2000
Promoter
Name: recAp
+1: 2823819
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 50
Sequence: cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc
                            -35                   -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Pages V., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: aaaagcaaaaGGGCCGCAGATGCGACCCttgtgtatca
Reference(s): [1] Pages V., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor recAp 2823829 2823848 -20.0 aaacacttgaTACTGTATGAGCATACAGTAtaattgcttc nd [BPP] [3], [4]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.0 2822657 2822692 ttgtttgataCACAAGGGTCGCATCTGCGGCCCTTTTGCTTTTTTaagttgtaag
  anti-terminator -10.3 2822678 2822746 ctctgtagatGATAGCGAAGGCGTAGCAGAAACTAACGAAGATTTTTAATCGTCTTGTTTGATACACAAGGGTCGCATctgcggccct
  anti-anti-terminator -9.5 2822733 2822767 cccgaactcaACGCCGGATTTCTCTGTAGATGATAGCGAAGGCGtagcagaaac
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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