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|
| Operon |  |
|---|---|
| Name: | recAX |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | recA |
| Synonym(s): | OP00086 |
| Gene(s): | recA Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | Based on the bacteriostatic antibiotic-induced persister model, 37 and 9 genes were found to produce defects in rifampin- and tetracycline-induced persister formation, respectively. Only six mutants were found to overlap in both treatment-induced persister screens: recA, recC, ruvA, uvrD, fis, and acrB. Four of these mutants (recA, recC, ruvA, and uvrD) mapped to the DNA repair pathway, one mutant mapped to a global transcriptional regulator (fis), and one to a gene for an efflux protein (acrB) Cui P, Niu H, Shi W, Zhang S, Zhang W, Zhang Y,2018 Other genes that mapped to transporters, membrane biogenesis, LPS biosynthesis, flagellum biosynthesis, metabolism (folate and energy), and translation were more specific to rifampin-induced persisters Cui P, Niu H, Shi W, Zhang S, Zhang W, Zhang Y,2018 A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for recA . This structure could regulate the expression of the gene, as observed for hemL gene expression Shao X, Zhang W, Umar MI, Wong HY, Seng Z, Xie Y, Zhang Y, Yang L, Kwok CK, Deng X,2020. |
| Evidence: | [LTED] Length of transcript experimentally determined |
| Reference(s): |
[1] Clark AJ., et al., 1994 [2] Fernandez De Henestrosa AR., et al., 2000 |
| Promoter | |
| Name: | recAp |
| +1: | 2823819 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 50 |
| Sequence: |
cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc -35 -10 +1 |
| Evidence: |
[HIPP] [ICWHO] [TIM] |
| Reference(s): |
[3] Huerta AM., et al., 2003 [4] Pages V., et al., 2003 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aaaagcaaaaGGGCCGCAGATGCGACCCttgtgtatca |
| Reference(s): | [4] Pages V., et al., 2003 |
| Name: | recAX |
| Gene(s): | recX, recA Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [ITCR] Inferred through co-regulation [LTED] Length of transcript experimentally determined |
| Reference(s): |
[4] Pages V., et al., 2003 [6] Stohl EA., et al., 2003 |
| Promoter | |
| Name: | recAp |
| +1: | 2823819 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 50 |
| Sequence: |
cttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttCaacagaacatattgactatc -35 -10 +1 |
| Evidence: |
[HIPP] [ICWHO] [TIM] |
| Reference(s): |
[3] Huerta AM., et al., 2003 [4] Pages V., et al., 2003 |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | reverse |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -16.0 | 2822657 | 2822692 | ttgtttgataCACAAGGGTCGCATCTGCGGCCCTTTTGCTTTTTTaagttgtaag | |
| anti-terminator | -10.3 | 2822678 | 2822746 | ctctgtagatGATAGCGAAGGCGTAGCAGAAACTAACGAAGATTTTTAATCGTCTTGTTTGATACACAAGGGTCGCATctgcggccct | |
| anti-anti-terminator | -9.5 | 2822733 | 2822767 | cccgaactcaACGCCGGATTTCTCTGTAGATGATAGCGAAGGCGtagcagaaac |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| Reference(s) |
|
|---|---|
[HIPP] Human inference of promoter position