RegulonDB RegulonDB 11.1: Operon Form
   

uvrA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: uvrA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: uvrA
Synonym(s): OP00097
Gene(s): uvrA   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Kenyon CJ., et al., 1981
Promoter
Name: uvrAp
+1: 4273936
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 65
Sequence: cactccgggtaatgcattccaatactgtatattcattcaggtcaatttgtgtcataattaAccgtttgtgatcgccggtag
                                     -35                  -1+1                   
Evidence: [COMP-AINF]
[COMP-HINF]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [2] Huerta AM., et al., 2003
[3] Ogasawara H., et al., 2005
[4] Sancar A., et al., 1982
Terminator(s)
Type: rho-independent
Sequence: ttgctggtgcAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCttaaggaagc
Reference(s): [5] Feng CQ., et al., 2019
[6] Lesnik EA., et al., 2001
TF binding sites (TFBSs)
[ArcA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArcA-phosphorylated repressor uvrAp 4273931 4273945 -2.5 tcaatttgtgTCATAATTAACCGTTtgtgatcgcc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [3]
proximal ArcA-phosphorylated repressor uvrAp 4273994 4274008 -65.5 ctggccagacATTGTTACACAACACtccgggtaat nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [3]
remote ArcA-phosphorylated repressor uvrAp 4274034 4274048 -105.5 cgcttttactATGTTGTGACCTCGGttccgggaaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [3]
remote ArcA-phosphorylated repressor uvrAp 4274077 4274091 -148.5 gttctgaataAGTGTAAACGCGCGAttgtaccatt nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [3]
[LexA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor uvrAp 4273955 4273974 -28.5 tgcattccaaTACTGTATATTCATTCAGGTcaatttgtgt nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] nd nd


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts


Reference(s)    

 [1] Kenyon CJ., Walker GC., 1981, Expression of the E. coli uvrA gene is inducible., Nature 289(5800):808-10

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Ogasawara H., Teramoto J., Yamamoto S., Hirao K., Yamamoto K., Ishihama A., Utsumi R., 2005, Negative regulation of DNA repair gene (uvrA) expression by ArcA/ArcB two-component system in Escherichia coli., FEMS Microbiol Lett 251(2):243-9

 [4] Sancar A., Sancar GB., Rupp WD., Little JW., Mount DW., 1982, LexA protein inhibits transcription of the E. coli uvrA gene in vitro., Nature 298(5869):96-8

 [5] Feng CQ., Zhang ZY., Zhu XJ., Lin Y., Chen W., Tang H., Lin H., 2019, iTerm-PseKNC: a sequence-based tool for predicting bacterial transcriptional terminators., Bioinformatics 35(9):1469-1477

 [6] Lesnik EA., Sampath R., Levene HB., Henderson TJ., McNeil JA., Ecker DJ., 2001, Prediction of rho-independent transcriptional terminators in Escherichia coli., Nucleic Acids Res 29(17):3583-94

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