RegulonDB RegulonDB 10.10: Operon Form
   

cysJIH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cysJIH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cysJIH
Synonym(s): OP00106, cysJHI
Gene(s): cysH, cysI, cysJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): cysJIH expression is inhibited by oleanolic and ursolic acid, which in turn affect the expression of the cysteine regulon and the stress response Grudniak AM,2011
Reference(s): [1] Ostrowski J., et al., 1989
Promoter
Name: cysJp
+1: 2891964
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 66
Sequence: ggttattagttatcgctatcccgtctttaatccacaccgtttgccccgttaaccttacctTctcttctgttttatgggcgc
                          -35                     -10       +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Ostrowski J., et al., 1989
TF binding sites (TFBSs)
[CysB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CysB-O-acetyl-L-serine activator cysJp 2891998 2892039 -55.0 attgctaaaaCAGGTTAGTCGATTTGGTTATTAGTTATCGCTATCCCGTCTTtaatccacac nd [APIORCISFBSCS] nd
[IHF,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote IHF activator cysJp 2892082 2892094 -124.0 gctaaattcaTTTGTTTTTCATTaggttggtta nd [GEA], [BPP] [4]


Transcription unit          
Name: cysH
Gene(s): cysH   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: cysHp
+1: Unknown
Evidence: [IEP]
Reference(s): [5] Zaslaver A., et al., 2006
TF binding sites (TFBSs)
[HypT,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd HypT-Met-oxidized activator cysHp nd nd nd nd nd [GEA], [BPP] [6], [7]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [IEP] Inferred from expression pattern


Reference(s)    

 [1] Ostrowski J., Wu JY., Rueger DC., Miller BE., Siegel LM., Kredich NM., 1989, Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase., J Biol Chem 264(26):15726-37

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Ostrowski J., Kredich NM., 1989, Molecular characterization of the cysJIH promoters of Salmonella typhimurium and Escherichia coli: regulation by cysB protein and N-acetyl-L-serine., J Bacteriol 171(1):130-40

 [4] Sirko A., Weglenska A., Hulanicka D., 1998, Integration host factor positively regulates cycJIH transcription., Mol Gen Genet 258(1-2):174-7

 [5] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8

 [6] Drazic A., Miura H., Peschek J., Le Y., Bach NC., Kriehuber T., Winter J., 2013, Methionine oxidation activates a transcription factor in response to oxidative stress., Proc Natl Acad Sci U S A 110(23):9493-8

 [7] Gebendorfer KM., Drazic A., Le Y., Gundlach J., Bepperling A., Kastenmuller A., Ganzinger KA., Braun N., Franzmann TM., Winter J., 2012, Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli., J Biol Chem 287(9):6892-903

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