RegulonDB RegulonDB 10.6.3: Operon Form
   

ilvIH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: ilvIH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: ilvIH
Gene(s): ilvI, ilvH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Blattner FR., et al., 1997
[2] Lago CT., et al., 1985
[3] Squires CH., et al., 1983
Promoter
Name: ilvIp4
+1: 85420
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 210
Sequence: ttttattgaatgtagaattttattctgaatgtgtgggctctctattttaggattaattaaAaaaatagagaaattgctgta
                         -35                    -10         +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [4] Haughn GW., et al., 1985


Transcription unit       
Name: ilvIH
Gene(s): ilvI, ilvH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Blattner FR., et al., 1997
[2] Lago CT., et al., 1985
[3] Squires CH., et al., 1983
Promoter
Name: ilvIp3
+1: 85453
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 177
Sequence: tgggctctctattttaggattaattaaaaaaatagagaaattgctgtaagttgtgggattCagccgatttattatcaattt
                        -35                    -10          +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [4] Haughn GW., et al., 1985


Transcription unit          
Name: ilvIH
Gene(s): ilvI, ilvH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Blattner FR., et al., 1997
[2] Lago CT., et al., 1985
[3] Squires CH., et al., 1983
Promoter
Name: ilvIp2
+1: 85540
Distance from start of the gene: 90
Sequence:
Evidence: [HIPP]
[IMP]
[TIM]
Reference(s): [4] Haughn GW., et al., 1985
[5] Willins DA., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Lrp repressor ilvIp2 85341 85355 -192.0 agacatgaatGTCTGGTTTATTCTGcattttttat nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
remote Lrp repressor ilvIp2 85372 85386 -161.0 ttattgaatgTAGAATTTTATTCTGaatgtgtggg nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp repressor ilvIp2 85453 85467 -80.0 ttgtgggattCAGCCGATTTATTATcaatttaatc nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp repressor ilvIp2 85488 85502 -45.0 ctctgtaatgGAGGATTTTATCGTTtcttttcacc nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp repressor ilvIp2 85517 85531 -16.0 ttcacctttcCTCCTGTTTATTCTTattaccccgt nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp repressor ilvIp2 85537 85551 5.0 ttcttattacCCCGTGTTTATGTCTctggctgcca nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition ilvIp2   [GEA] [14]
Evidence: [GEA] Gene expression analysis
Reference(s): [14] Baccigalupi L., et al., 1995


Transcription unit          
Name: ilvIH
Synonym(s): OP00110
Gene(s): ilvI, ilvH   Genome Browser M3D Gene expression COLOMBOS
Note(s): The ilvIH operon is activated by Lrp in a leucine-sensitive manner; activation is optimum in the absence of leucine, but it is reduced about 30-fold in its presence |CITS:[ 8429549]| and by the effects of HNS |CITS: [8152423]|. Lrp binds to six sites, four proximal tandem sites and two distal sites, at the upstream region of the ilvIH operon |CITS:[ 8429549]|. The binding of Lrp to the proximal sites is necessary for the activation of the operon, but it is not enough; Lrp must also bind to the distal sites in order to allow operon expression |CITS:[12218014]|.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Blattner FR., et al., 1997
[6] Jafri S., et al., 2002
[3] Squires CH., et al., 1983
[11] Wang Q., et al., 1993
Promoter
Name: ilvIHp1
+1: 85600
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: gtgtttatgtctctggctgccaattgcttaagcaagatcggacggttaatgtgttttacaCattttttccgtcaaacagtg
                        -35                    -10          +1                   
Evidence: [HIPP]
[TASES]
[TIM]
Reference(s): [4] Haughn GW., et al., 1985
[7] Marasco R., et al., 1994
[11] Wang Q., et al., 1993
[5] Willins DA., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS1 repressor ilvIHp1 nd nd nd nd nd [GEA] [15]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Lrp activator ilvIHp1 85341 85355 -252.0 agacatgaatGTCTGGTTTATTCTGcattttttat nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
remote Lrp activator ilvIHp1 85372 85386 -221.0 ttattgaatgTAGAATTTTATTCTGaatgtgtggg nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
remote Lrp activator ilvIHp1 85453 85467 -140.0 ttgtgggattCAGCCGATTTATTATcaatttaatc nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
remote Lrp activator ilvIHp1 85488 85502 -105.0 ctctgtaatgGAGGATTTTATCGTTtcttttcacc nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp activator ilvIHp1 85517 85531 -76.0 ttcacctttcCTCCTGTTTATTCTTattaccccgt nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
proximal Lrp activator ilvIHp1 85537 85551 -56.0 ttcttattacCCCGTGTTTATGTCTctggctgcca nd [BCE], [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9], [10], [11], [12], [13]
Note(s): 1It has been proven that HNS has regulatory effects for the ilvIH operon under high osmolarity conditions, decreasing the Lrp action; these effects superimpose the Lrp activation of the operon Levinthal M,19947It has been proven that HNS has regulatory effects for the ilvIH operon under high osmolarity conditions, decreasing the Lrp action; these effects superimpose the Lrp activation of the operon Levinthal M,1994


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  dsrA antisense post-transcriptional regulation repressor       [HIFS] [16]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [17] null null
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -6.9 85476 85498 tcaatttaatCCTCTGTAATGGAGGATTTTATcgtttctttt
  anti-terminator -4.8 85447 85479 gtaagttgtgGGATTCAGCCGATTTATTATCAATTTAATCCTctgtaatgga
  anti-anti-terminator -3.6 85430 85462 aaaatagagaAATTGCTGTAAGTTGTGGGATTCAGCCGATTTattatcaatt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Blattner FR., Plunkett G., Bloch CA., Perna NT., Burland V., Riley M., Collado-Vides J., Glasner JD., Rode CK., Mayhew GF., Gregor J., Davis NW., Kirkpatrick HA., Goeden MA., Rose DJ., Mau B., Shao Y., 1997, The complete genome sequence of Escherichia coli K-12., Science 277(5331):1453-74

 [2] Lago CT., Sannia G., Marino G., Squires CH., Calvo JM., De Felice M., 1985, The ilvIH operon of Escherichia coli K-12. Identification of the gene products and recognition of the translational start by polypeptide microsequencing., Biochim Biophys Acta 824(1):74-9

 [3] Squires CH., De Felice M., Devereux J., Calvo JM., 1983, Molecular structure of ilvIH and its evolutionary relationship to ilvG in Escherichia coli K12., Nucleic Acids Res 11(15):5299-313

 [4] Haughn GW., Squires CH., De Felice M., Largo CT., Calvo JM., 1985, Unusual organization of the ilvIH promoter of Escherichia coli., J Bacteriol 163(1):186-98

 [5] Willins DA., Calvo JM., 1992, In vitro transcription from the Escherichia coli ilvIH promoter., J Bacteriol 174(23):7648-55

 [6] Jafri S., Chen S., Calvo JM., 2002, ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA., J Bacteriol 184(19):5293-300

 [7] Marasco R., Varcamonti M., La Cara F., Ricca E., De Felice M., Sacco M., 1994, In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli., J Bacteriol 176(17):5197-201

 [8] Platko JV., Calvo JM., 1993, Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine., J Bacteriol 175(4):1110-7

 [9] Platko JV., Willins DA., Calvo JM., 1990, The ilvIH operon of Escherichia coli is positively regulated., J Bacteriol 172(8):4563-70

 [10] Ricca E., Aker DA., Calvo JM., 1989, A protein that binds to the regulatory region of the Escherichia coli ilvIH operon., J Bacteriol 171(3):1658-64

 [11] Wang Q., Calvo JM., 1993, Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH., J Mol Biol 229(2):306-18

 [12] Wang Q., Calvo JM., 1993, Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure., EMBO J 12(6):2495-501

 [13] Wang Q., Sacco M., Ricca E., Lago CT., De Felice M., Calvo JM., 1993, Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium., Mol Microbiol 7(6):883-91

 [14] Baccigalupi L., Marasco R., Ricca E., De Felice M., Sacco M., 1995, Control of ilvIH transcription during amino acid downshift in stringent and relaxed strains of Escherichia coli., FEMS Microbiol Lett 131(1):95-8

 [15] Levinthal M., Lejeune P., Danchin A., 1994, The H-NS protein modulates the activation of the ilvIH operon of Escherichia coli K12 by Lrp, the leucine regulatory protein., Mol Gen Genet 242(6):736-43

 [16] Jackson AL, Chen R, Loeb LA., 1998, Induction of microsatellite instability by oxidative DNA damage., Proc Natl Acad Sci U S A. Oct;95(21):12468-73.

 [17] null, null, null, null


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