RegulonDB RegulonDB 10.8: Operon Form
   

tyrB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: tyrB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: tyrB
Synonym(s): OP00123
Gene(s): tyrB   Genome Browser M3D Gene expression COLOMBOS
Note(s): When phenylalanine or tyrosine along with ATP interact with the TyrR protein, the transcription of the tyrB gene is repressed through the binding of aTyrR hexamer to a weak and strong TyrR box located 19.5 and 42.5 bp downstream of the tyrBp promoter, respectively Yang J,2002
The two aromatic amino acids affect the transcription in different ways. While tyrosine prevents the open complex formation, phenylalanine affects one or more steps after the open complex formationYang J,2002
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Fotheringham IG., et al., 1986
[2] Yang J., et al., 1987
Promoter
Name: tyrBp
+1: 4267082
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 32
Sequence: ttgccgttcgctcctgaacatccactcgatcttcgccttcttccggtttattgtgttttaAccacctgcccgtaaacctgg
                         -35                     -10        +1                   
Evidence: [CV(RPF)]
[CV(RPF/RS-EPT-CBR)]
[CV(RPF/TIM)]
[CV(RS-EPT-CBR)]
[CV(TIM)]
[HIPP]
[RPF]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Salgado H, et al., 2012
[4] Yang J., et al., 2002
[2] Yang J., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: atgcaggaaaGCAGGCTGGAGCTACCCAGCCTGCagtgaaatta
Reference(s): [1] Fotheringham IG., et al., 1986
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TyrR-tyrosine1 repressor tyrBp 4267092 4267109 19.5 accacctgccCGTAAACCTGGAGAACCAtcgcgtgttt nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [4]
remote TyrR-tyrosine2 repressor tyrBp 4267115 4267132 42.5 aaccatcgcgTGTTTCAAAAAGTTGACGcctacgctgg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [4]
Note(s): 1The TyrR-binding site at position bp 19.5 downstream of the promoter is a weak TyrR box that is involved in the repression of tyrB gene transcription. For this repression, it is necessary that TyrR, with tyrosine or phenylalanine, binds to this site and another strong site located 42.5 bp downstream from the promoter Yang J,2002
2The TyrR-binding site at position bp 42.5 downstream of the promoter is a strong TyrR box that is involved in repression of tyrB gene transcription. For this repression, it is necessary that TyrR, with tyrosine or phenylalanine, binds this site and another weak site located 19.5 bp downstream from the promoter Yang J,20021The TyrR-binding site at position bp 19.5 downstream of the promoter is a weak TyrR box that is involved in the repression of tyrB gene transcription. For this repression, it is necessary that TyrR, with tyrosine or phenylalanine, binds to this site and another strong site located 42.5 bp downstream from the promoter Yang J,2002
2The TyrR-binding site at position bp 42.5 downstream of the promoter is a strong TyrR box that is involved in repression of tyrB gene transcription. For this repression, it is necessary that TyrR, with tyrosine or phenylalanine, binds this site and another weak site located 19.5 bp downstream from the promoter Yang J,2002





RegulonDB