RegulonDB RegulonDB 10.10: Operon Form
   

purA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: purA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: purA
Synonym(s): OP00133
Gene(s): purA   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for purA . This structure could regulate the expression of the gene, as observed for hemL gene expression Shao X, Zhang W, Umar MI, Wong HY, Seng Z, Xie Y, Zhang Y, Yang L, Kwok CK, Deng X,2020.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Meng LM., et al., 1990
[2] Wolfe SA., et al., 1988
Promoter
Name: purAp
+1: 4404664
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 23
Sequence: ggtcatttttgagtgcaaaaagtgctgtaactctgaaaaagcgatggtagaatccattttTaagcaaacggtgattttgaa
                         -35                    -10         +1                   
Evidence: [ICWHO]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Salgado H, et al., 2012
[2] Wolfe SA., et al., 1988
TF binding sites (TFBSs)
[GadE,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GadE activator purAp nd nd nd nd nd [GEA], [BPP] [8]
[MarA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MarA repressor purAp 4404611 4404630 -43.0 cgaggtcattTTTGAGTGCAAAAAGTGCTGtaactctgaa nd [GEA], [APIORCISFBSCS], [BPP], [SM] [7]
[PurR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor purAp 4404566 4404581 -90.5 ctacatgttgAGGAAAACGATTGGCTgaacaaaaaa nd [GEA], [APIORCISFBSCS], [BPP] [5], [6]
proximal PurR-hypoxanthine repressor purAp 4404665 4404680 9.5 atccatttttAAGCAAACGGTGATTTtgaaaaatgg nd [GEA], [APIORCISFBSCS], [BPP] [5], [6]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [SM] Site mutation


Reference(s)    

 [1] Meng LM., Kilstrup M., Nygaard P., 1990, Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli., Eur J Biochem 187(2):373-9

 [2] Wolfe SA., Smith JM., 1988, Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12., J Biol Chem 263(35):19147-53

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64

 [6] He B., Zalkin H., 1994, Regulation of Escherichia coli purA by purine repressor, one component of a dual control mechanism., J Bacteriol 176(4):1009-13

 [7] Schneiders T., Barbosa TM., McMurry LM., Levy SB., 2004, The Escherichia coli transcriptional regulator MarA directly represses transcription of purA and hdeA., J Biol Chem 279(10):9037-42

 [8] Hommais F., Krin E., Coppee JY., Lacroix C., Yeramian E., Danchin A., Bertin P., 2004, GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli., Microbiology 150(Pt 1):61-72

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