RegulonDB RegulonDB 10.9: Operon Form
   

araJ operon and associated TUs in Escherichia coli K-12 genome


Transcription unit          
Name: araJ
Synonym(s): OP00139
Gene(s): araJ   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Reeder T., et al., 1991
Promoter
Name: araJp
+1: 412529
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 48
Sequence: gaatacagaggggcgaattatctcttggccttgctggtcgttatcctgcaagctatcactTtattggctacggtgattggt
                         -35                      -10       +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [2] Hendrickson W., et al., 1990
[3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[AraC,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal AraC-arabinose activator araJp 412564 412580 -43.0 atgaatacagAGGGGCGAATTATCTCTTggccttgctg nd [AIBSCS], [BPP] [2]
proximal AraC-arabinose activator araJp 412585 412601 -64.0 aaataactatTCAGCAGGATAATGAATAcagaggggcg nd [APIORCISFBSCS], [BPP] [2]
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator araJp 412609 412630 -90.5 acagcaaactGGAAAGTACGTTTGCAGTGAAATaactattcag nd [APIORCISFBSCS], [BPP] [2]
[CRP,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP repressor araJp 412475 412497 44.5 gccgttctggTGGTTGTGATGGTGGTATGAAAAaagtcatttt nd [APIORCISFBSCS], [BPP] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Reeder T., Schleif R., 1991, Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon., J Bacteriol 173(24):7765-71

 [2] Hendrickson W., Stoner C., Schleif R., 1990, Characterization of the Escherichia coli araFGH and araJ promoters., J Mol Biol 215(4):497-510

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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