RegulonDB RegulonDB 10.9: Operon Form
   

betT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: betT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: betT
Synonym(s): OP00156
Gene(s): betT   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Lamark T., et al., 1996
[2] Rkenes TP., et al., 1996
Promoter
Name: betTp
+1: 329421
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: tttatctattaaagcggttattgattggacgttcaatataaaatgtgtcttaattgttacGaatttgattttaaatagtaa
                         -35                        -10     +1                   
Note(s): This promoter displays similarity in the region upstream of the -10 box to the proU promoter, including a TG motif at -14 and -15. This motif is important for proU expression.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[1] Lamark T., et al., 1996
TF binding sites (TFBSs)
[ArcA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArcA-Phosphorylated1 repressor betTp 329416 329430 3.0 tgtcttaattGTTACGAATTTGATTTtaaatagtaa nd [BCE], [GEA] [5]
proximal ArcA-Phosphorylated repressor betTp 329428 329442 15.0 tacgaatttgATTTTAAATAGTAACAataacagtgg nd [BCE], [GEA] [1]
[BetI,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal BetI1 repressor betTp 329381 329400 -30.0 aaagcggttaTTGATTGGACGTTCAATATAAaatgtgtctt nd [APIORCISFBSCS], [BPP], [GEA], [GS] [1], [2], [4]
[Cra,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Cra activator betTp nd nd nd nd nd [BPP], [GEA], [IGI] [6]
Note(s): 1Several putative ArcA-binding sites with one or two mismatches are located within the betT promoter region.
1BetI is a repressor that regulates the betT promoter in response to the inducer choline.1BetI is a repressor that regulates the betT promoter in response to the inducer choline.
2Several putative ArcA-binding sites with one or two mismatches are located within the betT promoter region.


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [BCE] Binding of cellular extracts

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GS] genomic SELEX

 [IGI] Inferred from genetic interaction


Reference(s)    

 [1] Lamark T., Rokenes TP., McDougall J., Strom AR., 1996, The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress., J Bacteriol 178(6):1655-62

 [2] Rkenes TP., Lamark T., Strom AR., 1996, DNA-binding properties of the BetI repressor protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex formation., J Bacteriol 178(6):1663-70

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Shimada T., Ogasawara H., Ishihama A., 2018, Single-target regulators form a minor group of transcription factors in Escherichia coli K-12., Nucleic Acids Res 46(8):3921-3936

 [5] Lynch AS., Lin EC., 1996, Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters., J Bacteriol 178(21):6238-49

 [6] Son YJ., Phue JN., Trinh LB., Lee SJ., Shiloach J., 2011, The role of Cra in regulating acetate excretion and osmotic tolerance in E. coli K-12 and E. coli B at high density growth., Microb Cell Fact 10:52

RegulonDB