RegulonDB RegulonDB 10.6.3: Operon Form
   

cadC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cadC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cadC
Synonym(s): OP00158, cadBA
Gene(s): cadC   Genome Browser M3D Gene expression COLOMBOS
Note(s): The old name of this transcription unit in RegulonDB was cadCBA. With updated information we now describe two transcription units, cadC and cadBA.
Reference(s): [1] Takayama M., et al., 1994
[2] Watson N., et al., 1992
Promoter
Name: cadCp
+1: 4361963
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 29
Sequence: ataatatgtaaataaacccatctatagatggtaaaaataggttgtggcaattatcattgcAtcattcccttttcgaatgag
                            -10                     -35     +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [3] Neely MN., et al., 1996
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd CadC activator cadCp nd nd nd nd nd [GEA] [2]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote FNR activator cadCp 4362113 4362126 -156.5 atgatgaattTTGATGGTAAGGAAtattacttct nd [AIBSCS], [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor cadCp nd nd nd nd nd [BPP] [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd LeuO1 repressor cadCp nd nd nd nd nd [GEA] [7]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MlrA1 activator cadCp 4362148 4362182 -201.5 aagtgctccgGACAATTTTCCATGGATTATTTTCTGAACATTTTTctttggcaaa nd [BPP], [HIBSCS] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd OmpR1 repressor cadCp nd nd nd nd nd [BPP], [GEA] [8]
Note(s): 1It is likely that LeuO binds directly to cadC promoter sequences to reduce cadC gene expression Shi X,1995.1The MlrA-cadCp regulatory interaction was identified by sequence analysis reported by Ogasawara et al., and by a gel shift assay using purified transcription factor (data not provided) Ogasawara H,20101Under acid stress at pH 6.5, OmpR repressed the cadC gene, thus eliminating proton consumption and acidifying the cytoplasm Chakraborty S,2017
OmpR in its dimeric state binds DNA, but phosphorylation of OmpR is not required Chakraborty S,20171The MlrA-cadCp regulatory interaction was identified by sequence analysis reported by Ogasawara et al., and by a gel shift assay using purified transcription factor (data not provided) Ogasawara H,2010
5It is likely that LeuO binds directly to cadC promoter sequences to reduce cadC gene expression Shi X,1995.
6Under acid stress at pH 6.5, OmpR repressed the cadC gene, thus eliminating proton consumption and acidifying the cytoplasm Chakraborty S,2017
OmpR in its dimeric state binds DNA, but phosphorylation of OmpR is not required Chakraborty S,2017
External database links:
UNIPROT:
P23890




Reference(s)    

 [1] Takayama M., Ohyama T., Igarashi K., Kobayashi H., 1994, Escherichia coli cad operon functions as a supplier of carbon dioxide., Mol Microbiol 11(5):913-8

 [2] Watson N., Dunyak DS., Rosey EL., Slonczewski JL., Olson ER., 1992, Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH., J Bacteriol 174(2):530-40

 [3] Neely MN., Olson ER., 1996, Kinetics of expression of the Escherichia coli cad operon as a function of pH and lysine., J Bacteriol 178(18):5522-8

 [4] Ogasawara H., Yamamoto K., Ishihama A., 2010, Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli., FEMS Microbiol Lett 312(2):160-8

 [5] Constantinidou C., Hobman JL., Griffiths L., Patel MD., Penn CW., Cole JA., Overton TW., 2006, A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth., J Biol Chem 281(8):4802-15

 [6] Krin E., Danchin A., Soutourina O., 2010, Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli., BMC Microbiol 10:273

 [7] Shi X., Bennett GN., 1995, Effects of multicopy LeuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli., J Bacteriol 177(3):810-4

 [8] Chakraborty S., Winardhi RS., Morgan LK., Yan J., Kenney LJ., 2017, Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells., Nat Commun 8(1):1587


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