RegulonDB RegulonDB 10.10: Operon Form
   

malK-lamB-malM operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: malK-lamB-malM
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: malK-lamB-malM
Synonym(s): OP00165
Gene(s): malK, lamB, malM   Genome Browser M3D Gene expression COLOMBOS
Note(s): malK-lamB-malM, among other genes involved in carbon source transport and metabolism, were downregulated in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 1208335|.
Reference(s): [1] Rousset JP., et al., 1986
Promoter
Name: malKp
+1: 4246736
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 48
Sequence: ccataaaaaagccagggggtggaggatttaagccatctcctgatgacgcatagtcagcccAtcatgaatgttgctgtcgat
                           -35                   -10        +1                   
Evidence: [ICWHO]
[IMP]
[TASES]
Reference(s): [2] Huerta AM., et al., 2003
[3] Raibaud O., et al., 1989
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cyclic-AMP activator malKp 4246530 4246551 -195.5 caccgtcgctTTGTGTGATCTCTGTTACAGAAttggcggtaa nd [APIORCISFBSCS], [BPP], [SM] [4]
remote CRP-cyclic-AMP activator malKp 4246559 4246580 -166.5 gaattggcggTAATGTGGAGATGCGCACATAAaatcgccacg nd [AIBSCS], [APIORCISFBSCS], [BPP], [SM] [4]
remote CRP-cyclic-AMP activator malKp 4246593 4246614 -132.5 tcgccacgatTTTTGCAAGCAACATCACGAAAttccttacat nd [APIORCISFBSCS], [BPP], [SM] [4]
remote CRP-cyclic-AMP activator malKp 4246625 4246646 -100.5 ttccttacatGACCTCGGTTTAGTTCACAGAAgccgtgttct nd [AIBSCS], [BPP], [SM] nd
[MalT,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MalT-maltotriose-ATP activator malKp 4246501 4246510 -230.5 ctctttccatCCTCCTTGCCcctacgcccc nd [APIORCISFBSCS], [BPP], [SM] [4]
remote MalT-maltotriose-ATP activator malKp 4246511 4246520 -220.5 cctccttgccCCTACGCCCCaccgtcgctt nd [AIBSCS], [APIORCISFBSCS], [BPP], [SM] [4]
proximal MalT-maltotriose-ATP activator malKp 4246657 4246666 -74.5 gccgtgttctCATCCTCCCGcctcctcccc nd [AIBSCS], [BPP], [SM] nd
proximal MalT-maltotriose-ATP activator malKp 4246667 4246676 -64.5 catcctcccgCCTCCTCCCCcataaaaaag nd [APIORCISFBSCS], [BPP], [SM] nd
proximal MalT-maltotriose-ATP activator malKp 4246691 4246700 -40.5 aaaaagccagGGGGTGGAGGatttaagcca nd [APIORCISFBSCS], [BPP], [SM] [4]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -12.8 4246553 4246597 ttacagaattGGCGGTAATGTGGAGATGCGCACATAAAATCGCCACGATTTTTGcaagcaacat
  anti-terminator -13.7 4246521 4246559 ctacgccccaCCGTCGCTTTGTGTGATCTCTGTTACAGAATTGGCGGTaatgtggaga
  anti-anti-terminator -10.8 4246483 4246516 tttctttataCGGCAACCTCTTTCCATCCTCCTTGCCCCTACGccccaccgtc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [IMP] Inferred from mutant phenotype

 [TASES] Traceable author statement to experimental support

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [SM] Site mutation

 [AIBSCS] Automated inference based on similarity to consensus sequences


Reference(s)    

 [1] Rousset JP., Gilson E., Hofnung M., 1986, malM, a new gene of the maltose regulon in Escherichia coli K12. II. Mutations affecting the signal peptide of the MalM protein., J Mol Biol 191(3):313-20

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Raibaud O., Vidal-Ingigliardi D., Richet E., 1989, A complex nucleoprotein structure involved in activation of transcription of two divergent Escherichia coli promoters., J Mol Biol 205(3):471-85

 [4] Raibaud O., 1989, Nucleoprotein structures at positively regulated bacterial promoters: homology with replication origins and some hypotheses on the quaternary structure of the activator proteins in these complexes., Mol Microbiol 3(3):455-8

 [5] Gogol EB., Rhodius VA., Papenfort K., Vogel J., Gross CA., 2011, Small RNAs endow a transcriptional activator with essential repressor functions for single-tier control of a global stress regulon., Proc Natl Acad Sci U S A 108(31):12875-80

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