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| Operon |  |
|---|---|
| Name: | cysDNC |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | cysDNC |
| Synonym(s): | cysD, cysNC |
| Gene(s): | cysC, cysN, cysD Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | Originally, there were two operons, cysNC and cysD, described. In Leyh TS,1988, it was demonstrated that cysN and cysC were both contained in one operon. The evidence was the strong polarity between cysN and cysC, although no promoter was described. It was not determined whether the upstream gene (cysD) was part of the same operon, but the authors suggested that all the genes could be transcribed because their products are part of the same metabolic pathway. In a more recent paper, Malo MS,1990, a promoter upstream of cysD was described. In a third reference, Leyh TS,1992, it is proposed that the three genes are translationally coupled, confirming that they reside within the same operon. Additionally, a terminator of transcription has also been identified. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified [IEP] Inferred from expression pattern [ITC] Inferred through co-regulation [PM] Polar mutation |
| Reference(s): |
[1] Leyh TS., et al., 1988 [2] Leyh TS., et al., 1992 [3] Malo MS., et al., 1990 |
| Promoter | |
| Name: | cysDp |
| +1: | 2876368 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 39 |
| Sequence: |
aactactcattctaattggtaatttcattcgttctcttacgctccctatagtcgaaacatCtgatggcaagaaaatagcgg -35 -10 +1 |
| Note(s): | Originally, there were two promoters, cysNC and cysD, because there were two transcription units, cysD and cysNC in the database. With the current information, there is only one transcription unit, cysDNC, with three genes. See the note for transcription unit cysDNC. |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): | [3] Malo MS., et al., 1990 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | cgcaaataacCAGGAGATAAAACCGACCACGGCACCAGGCAGTGACCATGTGGTTTCTTCAtcctcagtaa |
| Reference(s): | [2] Leyh TS., et al., 1992 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CysB-O-acetyl-L-serine1 | activator | cysDp | 2876432 | 2876473 | -85.0 | cggtgccttaAGCACTTTTTGATATTAGCTTTGCCAAATCGTTATTCCGTTAaggaactact | nd | [BCE] | [2], [4] |
| Note(s): | 1The protein CysB regulates the expression of the cysDNC operon. This protein was previously indicated as involved in regulating the cydD and cysNC promoters. But the cysNC promoter has been eliminated from the database. See the note for the cysD promoter and the note for the transcription unit cysDNC.1The protein CysB regulates the expression of the cysDNC operon. This protein was previously indicated as involved in regulating the cydD and cysNC promoters. But the cysNC promoter has been eliminated from the database. See the note for the cysD promoter and the note for the transcription unit cysDNC. |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position