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| Operon |  |
|---|---|
| Name: | nupC |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | nupC |
| Synonym(s): | OP00198 |
| Gene(s): | nupC Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The nupC gene is regulated by Nac, CRP, and CytR. Two CRP-binding sites have been identified at -40.5 and -89.5 bp upstream of the transcriptional start site. Since no CytR-binding site has been identifed, it was suggested that the CytR protein could bind to a region between the two sites of CRP, such as in the regulatory regions of the deo and cdd operons Craig JE,1994. |
| Reference(s): | [1] Craig JE., et al., 1994 |
| Promoter | |
| Name: | nupCp |
| +1: | 2513009 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 33 |
| Sequence: |
acgtcattatagtgtgtgtcagatctcgttttccttaaccatgttacatagaatgtgcacGgaaatttaacctgcctcata -35 -10 +1 |
| Note(s): | The -10 and -35 regions of the nupCp promoter have been identified with the sequences TAGAAT and TCTCGT, respectively. There is a CRP-binding site overlapping the -35 region Craig JE,1994. |
| Evidence: |
[CV(RS-EPT-CBR/TA)] [CV(TA/TIM)] [HIPP] [RS-EPT-CBR] [TIM] |
| Reference(s): |
[1] Craig JE., et al., 1994 [2] Salgado H, et al., 2012 [3] Valentin-Hansen P., et al., 1991 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CRP-cAMP | activator | nupCp | 2512909 | 2512930 | -89.5 | aacatagcagAAATGTATGACAGATCACTATTtttgaagcct | nd | [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] | [1], [4] |
| proximal | CRP-cAMP | activator | nupCp | 2512958 | 2512980 | -40.5 | gacgtcattaTAGTGTGTGTCAGATCTCGTTTTccttaaccat | nd | [AIBSCS], [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] | [1], [3], [4], [5] |
| Note(s): | 1Although no CytR-binding sites have been identified, it was suggested that the CytR protein could bind cooperatively to a region between the two CRP-binding sites, as in the regulatory regions of the deo and cdd operons Craig JE,1994.4Although no CytR-binding sites have been identified, it was suggested that the CytR protein could bind cooperatively to a region between the two CRP-binding sites, as in the regulatory regions of the deo and cdd operons Craig JE,1994. |
| Reference(s) |
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|---|---|
[CV(RS-EPT-CBR/TA)] cross validation(RS-EPT-CBR/TA)