RegulonDB RegulonDB 10.8: Operon Form
   

exuT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: exuT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: exuT
Synonym(s): OP00201
Gene(s): exuT   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[IMP] Inferred from mutant phenotype
Reference(s): [1] Hugouvieux-Cotte-Pattat N., et al., 1982
[2] Mata-Gilsinger M., et al., 1983
[3] Mata-Gilsinger M., et al., 1983
[4] Nemoz G., et al., 1976
Promoter
Name: exuTp2
+1: 3245002
Distance from start of the gene: 222
Sequence: atattcccacatttgtgatggctctcaccttttaaagttgtatgacaagttatctttctgCcgtcgcaaatcataagtcga
Evidence: [TIM]
Reference(s): [5] Yamamoto K., et al., 2008
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator exuTp2 3244830 3244851 -161.5 ccgaagatatTTTCGTGAGTTAGATCAATAAAcgtagttaaa nd [AIBSCS] [6]
proximal CRP-cAMP activator exuTp2 3244952 3244974 -39.5 atattcccacATTTGTGATGGCTCTCACCTTTTaaagttgtat nd [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [GEA] [6], [7], [8]
[DpiA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DpiA-Pasp activator exuTp2 nd nd nd nd nd [BPP], [GEA] [5]
[ExuR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ExuR repressor exuTp2 3244850 3244867 -143.5 tagatcaataAACGTAGTTAAAAAAATTactctcaaag nd [AIBSCS] [6], [7]
proximal ExuR repressor exuTp2 3244976 3244993 -17.5 tcaccttttaAAGTTGTATGACAAGTTAtctttctgcc nd [AIBSCS], [CV(GEA)], [GEA] [3], [6]


Transcription unit          
Name: exuT
Gene(s): exuT   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: exuTp1
+1: 3245043
Distance from start of the gene: 181
Sequence: atgacaagttatctttctgccgtcgcaaatcataagtcgacggaatgcaaattgccgattCattcatttgttagatgaatc
Evidence: [TIM]
Reference(s): [5] Yamamoto K., et al., 2008
TF binding sites (TFBSs)
[DpiA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DpiA-Pasp activator exuTp1 nd nd nd nd nd [BPP], [GEA] [5]


Evidence    

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Hugouvieux-Cotte-Pattat N., Robert-Baudouy J., 1982, Determination of the transcription direction of the exuT gene in Escherichia coli K-12: divergent transcription of the exuT-uxaCA operons., J Bacteriol 151(1):480-4

 [2] Mata-Gilsinger M., Ritzenthaler P., 1983, Physical mapping of the exuT and uxaC operators by use of exu plasmids and generation of deletion mutants in vitro., J Bacteriol 155(3):973-82

 [3] Mata-Gilsinger M., Ritzenthaler P., Blanco C., 1983, Characterization of the operator sites of the exu regulon in Escherichia coli K-12 by operator-constitutive mutations and repressor titration., Genetics 105(4):829-42

 [4] Nemoz G., Robert-Baudouy J., Stoeber F., 1976, Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli., J Bacteriol 127(2):706-18

 [5] Yamamoto K., Matsumoto F., Oshima T., Fujita N., Ogasawara N., Ishihama A., 2008, Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli., Biosci Biotechnol Biochem 72(11):3011-4

 [6] Rodionov DA., Mironov AA., Rakhmaninova AB., Gelfand MS., 2000, Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria., Mol Microbiol 38(4):673-83

 [7] Medina-Rivera A., Abreu-Goodger C., Thomas-Chollier M., Salgado H., Collado-Vides J., van Helden J., 2011, Theoretical and empirical quality assessment of transcription factor-binding motifs., Nucleic Acids Res 39(3):808-24

 [8] Tsai MJ., Wang JR., Yang CD., Kao KC., Huang WL., Huang HY., Tseng CP., Huang HD., Ho SY., 2018, PredCRP: predicting and analysing the regulatory roles of CRP from its binding sites in Escherichia coli., Sci Rep 8(1):951

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