RegulonDB RegulonDB 10.7: Operon Form
   

narXL operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: narXL
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: narXL
Synonym(s): OP00213, narL
Gene(s): narL, narX   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Darwin AJ., et al., 1995
[2] Stewart V., et al., 1988
Promoter
Name: narXp
+1: 1277777
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 159
Sequence: gtgagtacagtgacttcataaaaattatgagatttttcacggtgctgtaaaatccctaccCttaccgatgtaaagcgacta
                           -35                  -10         +1                   
Evidence: [HIPP]
Reference(s): [3] Nohno T., et al., 1989
TF binding sites (TFBSs)
[FNR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote FNR repressor narXp 1277664 1277677 107.5 ccattccagaATGTATCGTCACATtcattaaggt nd [APIORCISFBSCS], [BCE] [5]
proximal FNR repressor narXp 1277845 1277858 -75.0 tatctatcatTGATATTTATCATTacccatagtg nd [APIORCISFBSCS], [BPP] [5]
remote FNR repressor narXp 1277877 1277890 -106.5 aatttgatgtAAATCAAACGATAAgataacttta nd [APIORCISFBSCS], [BCE] [3], [5]
[ModE,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ModE-MoO42-1 activator narXp 1277907 1277930 -141.5 cgtttcgatgTCTGCCACCTTAGTGTCTGTAGCTaaaggcaatt nd [APIORCISFBSCS], [BPP], [GEA] [4]
Note(s): 1A model has been proposed in which ModE-molybdate serves as a secondary transcriptional activator of both the hyc and narXL operons, which are activated primarily by the transcriptional activators FhlA and NarL, respectively.1A model has been proposed in which ModE-molybdate serves as a secondary transcriptional activator of both the hyc and narXL operons, which are activated primarily by the transcriptional activators FhlA and NarL, respectively.


Evidence    

 [HIPP] Human inference of promoter position

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Darwin AJ., Stewart V., 1995, Expression of the narX, narL, narP, and narQ genes of Escherichia coli K-12: regulation of the regulators., J Bacteriol 177(13):3865-9

 [2] Stewart V., Parales J., 1988, Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12., J Bacteriol 170(4):1589-97

 [3] Nohno T., Noji S., Taniguchi S., Saito T., 1989, The narX and narL genes encoding the nitrate-sensing regulators of Escherichia coli are homologous to a family of prokaryotic two-component regulatory genes., Nucleic Acids Res 17(8):2947-57

 [4] Self WT., Grunden AM., Hasona A., Shanmugam KT., 1999, Transcriptional regulation of molybdoenzyme synthesis in Escherichia coli in response to molybdenum: ModE-molybdate, a repressor of the modABCD (molybdate transport) operon is a secondary transcriptional activator for the hyc and nar operons., Microbiology 145 ( Pt 1):41-55

 [5] Takahashi K., Hattori T., Nakanishi T., Nohno T., Fujita N., Ishihama A., Taniguchi S., 1994, Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein., FEBS Lett 340(1-2):59-64

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