|Gene(s):||lysP Genome Browser M3D Gene expression COLOMBOS|
|Note(s):||In 2011 Ruiz et al. proposed that ArgP binds to a region of 15 bp (ATGAAGGTGTCTTAT) in the intergenic region of lysP Ruiz J,2011. DNase I digestion patterns showed that ArgP binds approximately 50 bp upstream of the transcription start site of lysP Ruiz J,2011. According to this evidence, and electrophoretic mobility shift assay results Ruiz J,2011. Marbaniang CN,2011, the curator assigned two possible binding sites of 20 bp for ArgP, located at -62.5 and -82.5 relative to the transcription start site of lysP.
The curator identified the consensus sequence of ArgP and assigned the possible central position of these binding sites, based on similarity to consensus sequences. The sequence proposed by Ruiz and colleagues is included in the binding site located at -62.5 Ruiz J,2011.
ArgP has been identified as binding as a dimer of dimers in the intergenic region of dnaA in E. coli Lee Y,1997 and also in the regulatory region of gdhA of Klebsiella pneumoniae 18424527. For this reason, there is a possibility that this regulator transcriptionally regulates as a dimer of dimers.
|Evidence:||[ICWHO] Inferred computationally without human oversight|
|Sigma Factor:||Sigma70 Sigmulon|
|Distance from start of the gene:||28|
-35 -10 +1
|Reference(s):|| Neely MN., et al., 1996|
|Type||Transcription factor||Function||Promoter||Binding Sites||Growth Conditions||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|proximal||ArgP||activator||lysPp||2248613||2248632||-62.5||taatccctggGCGATCATGAAGGTGTCTTAtaaccgtgta||nd||[APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IC], [SM]||, , |
|proximal||ArgP2||activator||lysPp||2248633||2248652||-82.5||gagattggctGCTTTACTTATAATCCCTGGgcgatcatga||nd||[APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IC], [SM]||, , |
|Transcription factor||Function||Promoter||Binding Sites||Evidence (Confirmed, Strong, Weak)||Reference(s)|
|ArgP1||activator||lysPp||2248633||2248652||-82.5||gagattggctGCTTTACTTATAATCCCTGGgcgatcatga||[APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IC], [SM]||, , |
|ArgP||activator||lysPp||2248613||2248632||-62.5||taatccctggGCGATCATGAAGGTGTCTTAtaaccgtgta||[APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IC], [SM]||, , |
 Nguyen Le Minh P., Velazquez Ruiz C., Vandermeeren S., Abwoyo P., Bervoets I., Charlier D., 2018, Differential protein-DNA contacts for activation and repression by ArgP, a LysR-type (LTTR) transcriptional regulator in Escherichia coli., Microbiol Res 206:141-158
 Ruiz J., Haneburger I., Jung K., 2011, Identification of ArgP and Lrp as transcriptional regulators of lysP, the gene encoding the specific lysine permease of Escherichia coli., J Bacteriol 193(10):2536-48