RegulonDB RegulonDB 10.8: Operon Form
   

alaS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: alaS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: alaS
Gene(s): alaS   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for alaS . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Putney SD., et al., 1981
Promoter
Name: alaSp
+1: 2822090
Distance from start of the gene: 79
Sequence: tttgccgactgaacgcatacggtattttacttcccagtcaagaaaacttatcttattcccActtttcagttaccagcccgg
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Putney SD., et al., 1981
[1] Putney SD., et al., 1981
[3] Salgado H, et al., 2012
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal AlaS-L-alanine1 repressor alaSp 2822080 2822114 -6.5 ttacttcccaGTCAAGAAAACTTATCTTATTCCCACTTTTCAGTTaccagcccgg nd [BPP], [CV(SM)], [GEA], [SM] [1], [4]
Note(s): 1To date, the alaRS operon is the only reported aminoacyl-tRNA synthetase (aaRS) that can regulate its own gene transcription Banerjee B,2019
Based on several biophysical characterizations of the alaRS-DNA binding site, it was shown that full-length alanyl-tRNA synthetase undergoes a conformational change in the presence of its promoter DNA, leading to formation of higher-order structures Banerjee B,20191To date, the alaRS operon is the only reported aminoacyl-tRNA synthetase (aaRS) that can regulate its own gene transcription Banerjee B,2019
Based on several biophysical characterizations of the alaRS-DNA binding site, it was shown that full-length alanyl-tRNA synthetase undergoes a conformational change in the presence of its promoter DNA, leading to formation of higher-order structures Banerjee B,2019





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