RegulonDB RegulonDB 10.8: Operon Form
   

katE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: katE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: katE
Gene(s): katE   Genome Browser M3D Gene expression COLOMBOS
Note(s): The first characterization for this transcription unit was done by von Ossowski I,1991 in 1991; its transcriptional start site was determined to be -123 bp upstream the ATG codon, and the investigators proposed a promoter sequence for σ38. This promoter sequence does not agree with the later-determined consensus for σ38 (based on several more promoters known to be σ38 affected), so in 1997 at Tanaka K,1997 the transcriptional start site for the katE transcription unit was restudied, and it was found to be a unique transcript that begins at -53/-54 bp upstream of the start codon, together with a really strong -10 box for the σ38 factor, very close to the consensus. In accordance with these new data, the +1 position and promoter sequence for katE transcription unit have been actualized here.
Jung IL,2003demonstrated that both rpoS and katE genes, which codify a transcriptional regulator and a product essential for the detoxification against H2O2-induced stress, respectively, were absolutely dependent on polyamines during entry into the stationary phase. These data suggest that polyamines could be directly participating in the defense mechanism against oxidative stress.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Schellhorn HE., et al., 1988
[2] Tanaka K., et al., 1997
[3] von Ossowski I., et al., 1991
Promoter
Name: katEp
+1: 1813814
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 53
Sequence: aacaccgtttccagaatagtctccgaagcgggatctggctggtggtctatagttagagagTtttttgaccaaaacagcggc
                       -35                 -10              +1                   
Note(s): The promoter sequence and start transcription site for the katE gene were originally reported by von Ossowski I,1991 but faced with a strange σ38 sequence that was not similar to its consensus, the promoter sequence was recharacterized. Tanaka K,1997found a unique +1 start transcription site, closer to the start codon of the gene (at bp -53) and a -10 promoter sequence (cTATAGT) very similar to the consensus for σ38 (TATAAT, described by 8475100.
Evidence: [IMP]
[TIM]
Reference(s): [2] Tanaka K., et al., 1997
[3] von Ossowski I., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: aaacacgtagGCCTGATAAGCGAAGCGCATCAGGCagttttgcgt
Reference(s): [3] von Ossowski I., et al., 1991
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis repressor katEp 1813809 1813823 3.0 tctatagttaGAGAGTTTTTTGACCaaaacagcgg nd [AIBSCS], [CV(GEA)], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Fur-Fe2+ activator katEp nd nd nd nd nd [IEP] [5]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -6.4 1813787 1813820 gaagcgggatCTGGCTGGTGGTCTATAGTTAGAGAGTTTTTTGaccaaaacag
  anti-terminator -12.8 1813756 1813797 atttcataacACCGTTTCCAGAATAGTCTCCGAAGCGGGATCTGGCTGGTGgtctatagtt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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