RegulonDB RegulonDB 10.7: Operon Form
   

srlAEBD-gutM-srlR-gutQ operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: srlAEBD-gutM-srlR-gutQ
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: srlAEBD-gutM-srlR-gutQ
Synonym(s): OP00177, gutABDMR, srlABDMR
Gene(s): srlA, srlE, srlB, srlD, gutM, srlR, gutQ   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] McEntee K. 1977
[2] Yamada M., et al., 1988
[3] Yamada M., et al., 1987
Promoter
Name: srlAp
+1: 2825791
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 41
Sequence: atctttcattttgcgatcaaaataacacttttaaatctttcaatctgattagattaggttGccgtttggtaataaaacaat
                             -35                  -10       +1                   
Note(s): Based on the one-round PCR product method, a novel site-directed mutagenesis method, it was determined that an srlA-driven expression system is a tool applicable for strong expression of foreign genes under cultivation conditions in LB medium without induction Nakamura M, Aihara J, Hoshida H, Akada R,2018 In addition, it was determined that the sequence adjacent to the start codon of the srlA gene is essential for high expression and that its promoter region is 100 bp. srlAp is a constitutive promoter in LB medium, and it is a sorbitol-enhanceable and glucose-, fructose-, galactose-, mannose-, xylose-, and arabinose- repressible promoter Nakamura M, Aihara J, Hoshida H, Akada R,2018
Evidence: [TIM]
Reference(s): [2] Yamada M., et al., 1988
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator srlAp 2825438 2825459 -342.0 atctgcatcaCATTGTGCTGCGGCTCAAGCAGgaagccgcca nd [AIBSCS], [GEA] [2], [4]
proximal CRP-cAMP activator srlAp 2825739 2825760 -41.5 ttatctttcaTTTTGCGATCAAAATAACACTTttaaatcttt nd [AIBSCS], [GEA] [2], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GutM activator srlAp nd nd nd nd nd [GEA] [2]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS activator srlAp nd nd nd nd nd [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd SrlR repressor srlAp nd nd nd nd nd [GEA] [2]
External database links:
UNIPROT:
P15081


Transcription unit       
Name: srlR
Synonym(s): gutR
Gene(s): srlR   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [2] Yamada M., et al., 1988
Promoter
Name: srlRp
+1: 2828860
Distance from start of the gene: 187
Sequence: cgatcagcagcgcatcgtcgacaccttgtttatgaaaggactgaccgtcttcgcccgaccGcaaaaaattcccgcaattac
Note(s): The srlR gene has its own promoter, but expression of this gene is primarily due to readthrough from the slr operon. The putative srlR promoter sequence may be TCTTCG, as the -10 sequence and TTGTTT as the -35 sequence.
Evidence: [HIPP]
[IMP]
[TIM]
Reference(s): [2] Yamada M., et al., 1988


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  gcvB unknown unknown repressor       [IMP] [1]
[6]
  gcvB unknown unknown repressor       [IMP] [6]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"





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