RegulonDB RegulonDB 10.8: Operon Form
   

argR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: argR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: argR
Synonym(s): OP00009, TU00007, argR_carAB
Gene(s): argR   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Cunin R., et al., 1983
Promoter
Name: argRp2
+1: 3384602
Distance from start of the gene: 101
Sequence: gaatttgcatgccgtgacgcaggcatgtttctcaataacgaaatttgataaaatcccgctCtttcataacattatttcagc
Evidence: [TIM]
Reference(s): [2] Lim DB., et al., 1987


Transcription unit          
Name: argR
Synonym(s): OP00009, argR_carAB
Gene(s): argR   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Cunin R., et al., 1983
Promoter
Name: argRp1
+1: 3384677
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 26
Sequence: ttcagccttcttcagggctgactgtttgcataaaaattcatctgtatgcacaataatgttGtatcaaccaccatatcgggt
                          -35                       -10     +1                   
Evidence: [AIPP]
[TIM]
Reference(s): [2] Lim DB., et al., 1987
TF binding sites (TFBSs)
[ArgR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArgR-L-arginine repressor argRp1 3384641 3384658 -27.5 gggctgactgTTTGCATAAAAATTCATCtgtatgcaca nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [3], [4], [5], [6]
proximal ArgR-L-arginine repressor argRp1 3384661 3384678 -7.5 aattcatctgTATGCACAATAATGTTGTatcaaccacc nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [3], [4], [5], [6]


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  dsrA antisense post-transcriptional regulation repressor      
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"


Evidence    

 [TIM] Transcription initiation mapping

 [AIPP] Automated inference of promoter position

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Cunin R., Eckhardt T., Piette J., Boyen A., Pierard A., Glansdorff N., 1983, Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12., Nucleic Acids Res 11(15):5007-19

 [2] Lim DB., Oppenheim JD., Eckhardt T., Maas WK., 1987, Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor., Proc Natl Acad Sci U S A 84(19):6697-701

 [3] Caldara M., Charlier D., Cunin R., 2006, The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation., Microbiology 152(Pt 11):3343-54

 [4] Charlier D., Roovers M., Van Vliet F., Boyen A., Cunin R., Nakamura Y., Glansdorff N., Pierard A., 1992, Arginine regulon of Escherichia coli K-12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression., J Mol Biol 226(2):367-86

 [5] Levitt M., 1992, Accurate modeling of protein conformation by automatic segment matching., J Mol Biol 226(2):507-33

 [6] Makarova KS., Mironov AA., Gelfand MS., 2001, Conservation of the binding site for the arginine repressor in all bacterial lineages., Genome Biol 2(4):RESEARCH0013

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