RegulonDB RegulonDB 10.8: Operon Form
   

nrdDG operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: nrdDG
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: nrdDG
Gene(s): nrdG, nrdD   Genome Browser M3D Gene expression COLOMBOS
Note(s): FNR is responsible for anaerobic activation of the nrdDG operon. Both sites of FNR (-64.5 and -33.5) have an additive effect in coordinating nrdDG operon expression in response to shifting oxygen concentrations Roca I,2008
H-NS represses expression of the class Ia (nrdAB operon) and class III (nrdDG operon) ribonucleotide reductases (RNRs) both in aerobically and in anaerobically growing cells. Under aerobic conditions, repression occurs during the exponential phase of growth as well as at the transition from the exponential to the stationary phase, a period when no dNTPs are needed. Under anoxic conditions, repression occurs mainly in exponentially growing cells Cendra Mdel M,2013
Evidence: [ITC] Inferred through co-regulation
Reference(s): [1] Garriga X., et al., 1996
Promoter
Name: nrdDp
+1: 4462838
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 178
Sequence: caaaaaaagctcaaacatccttgatgcaaagcactatatatagactttaaaatgcgtcccAacccaatatgttgtattaat
                     -35                        -10         +1                   
Note(s):

Evidence: [TIM]
Reference(s): [2] Boston T., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FNR1 activator nrdDp 4462865 4462878 -33.5 tcaaacatccTTGATGCAAAGCACtatatataga nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [3]
proximal FNR2 activator nrdDp 4462896 4462909 -64.5 gctttttactTTGAGCTACATCAAaaaaagctca nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor nrdDp nd nd nd nd nd [BPP], [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NrdR-Zn2+ repressor nrdDp 4462822 4462837 9.5 atgcgtcccaACCCAATATGTTGTATtaatcgacta nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [4]
proximal NrdR-Zn2+ repressor nrdDp 4462853 4462868 -22.5 ttgatgcaaaGCACTATATATAGACTttaaaatgcg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [4]
Note(s): 1The operon nrdDG is induced under anaerobiosis in the stationary phase, and this induction is FNR dependent.
2The operon nrdDG is induced under anaerobiosis in the stationary phase, and this induction is FNR dependent.1The operon nrdDG is induced under anaerobiosis in the stationary phase, and this induction is FNR dependent.
2The operon nrdDG is induced under anaerobiosis in the stationary phase, and this induction is FNR dependent.


Transcription unit       
Name: nrdG
Gene(s): nrdG   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [6] Zaslaver A., et al., 2006
Promoter
Name: nrdGp
+1: Unknown
Evidence: [IEP]
Reference(s): [6] Zaslaver A., et al., 2006





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