RegulonDB RegulonDB 10.6.3: Operon Form
   

exuR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: exuR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: exuR
Gene(s): exuR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype
Reference(s): [1] Hugovieux-Cotte-Pattat N., et al., 1982
Promoter
Name: exuRp2
+1: 3246618
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 34
Sequence: ccttctcaggcggctttttcatcactgcgagtagagctaaactcgcaaaagtggtataacAaatatagtctgccgtatcat
Evidence: [TIM]
Reference(s): [2] Tutukina MN., et al., 2016
TF binding sites (TFBSs)
[CRP,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP1 repressor exuRp2 3246482 3246500 -127.0 gcgattgaggTGGCGCAGGAAACACATAAcgatcccgca nd [GEA], [HIBSCS] [2]
[ExuR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ExuR repressor exuRp2 3246416 3246433 -193.5 gcagttctggCAGTGTTCGACCTGTTAGgtgcgctggt nd [BPP], [GEA], [HIBSCS] [2]
proximal ExuR repressor exuRp2 3246605 3246622 -4.5 taaactcgcaAAAGTGGTATAACAAATAtagtctgccg nd [BPP], [GEA], [HIBSCS] [1], [2], [3]
[UxuR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote UxuR repressor exuRp2 3246416 3246433 -193.5 gcagttctggCAGTGTTCGACCTGTTAGgtgcgctggt nd [BPP], [GEA], [HIBSCS] [2]
Note(s): 1CRP is required for exuR transcription in a sugar-independent manner, irrespective of whether the bacteria are grown with glucose or glucuronate as the main carbon source Tutukina MN,2016, and this might be due to its cAMP-independent binding to a nonspecific site leading to only a secondary effect Kolb A, Busby S, Buc H, Garges S, Adhya S,1993.3CRP is required for exuR transcription in a sugar-independent manner, irrespective of whether the bacteria are grown with glucose or glucuronate as the main carbon source Tutukina MN,2016, and this might be due to its cAMP-independent binding to a nonspecific site leading to only a secondary effect Kolb A, Busby S, Buc H, Garges S, Adhya S,1993.


Evidence    

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Hugovieux-Cotte-Pattat N., Robert-Baudouy J., 1982, Regulation and transcription direction of exuR, a self-regulated repressor in escherichia coli K-12., J Mol Biol 156(1):221-8

 [2] Tutukina MN., Potapova AV., Cole JA., Ozoline ON., 2016, Control of hexuronate metabolism in Escherichia coli by the two interdependent regulators, ExuR and UxuR: derepression by heterodimer formation., Microbiology 162(7):1220-31

 [3] Rodionov DA., Mironov AA., Rakhmaninova AB., Gelfand MS., 2000, Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria., Mol Microbiol 38(4):673-83

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