RegulonDB RegulonDB 10.6.3: Operon Form
   

hyfABCDEFGHIJR-focB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hyfABCDEFGHIJR-focB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: hyfABCDEFGHIJR-focB
Gene(s): hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, focB   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon is apparently silent, because it was not expressed at a significant level in a wild-type strain under the tested conditions |CITS: [9387241]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[IEP] Inferred from expression pattern
[ITCR] Inferred through co-regulation
Reference(s): [1] Andrews SC., et al., 1997
Promoter
Name: hyfAp
+1: 2601171
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 30
Sequence: cataacttattgaatatattgagttaatcagaatggcatcctttatgcaatatgaaatgcAatgtttcatatcattttcaa
                                  -24         -12           +1                   
Evidence: [HIPP]
[TIM] ; Method: Primer Extension
[TIM]
Reference(s): [2] Skibinski DA., et al., 2002
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP1 activator hyfAp 2601000 2601022 -160.5 atacctcactTCTCGTGATCAAGATCACATTCTcgctttcccc nd [AIBSCS], [GEA], [HIBSCS] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote FNR1 repressor hyfAp 2601059 2601072 -105.5 aatccattttTTGCTGAACGTTAAtgaccatcat nd [GEA], [HIBSCS] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote FhlA-formate1 activator hyfAp 2601034 2601047 -130.0 gctttcccctGCGACACGGGTGTCgaatccattt nd [GEA] [2], [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote HyfR1 activator hyfAp 2601033 2601050 -129.5 cgctttccccTGCGACACGGGTGTCGAAtccatttttt nd [AIBSCS], [GEA] [2], [3]
Note(s): 1CRP and anaerobiosis are required for the hyf operon expression when the HyfR protein is the activator Self WT,2004.1It seems that Fnr repress the hyf operon expression when it is activated by HyfR. Its is possible that binding of FNR may minimize the binding of HyfR, whose binding site is only 25 bases upstream of the FNR-binding site Self WT,2004.1Only FhlA mutants can activate the transcription of the hyf operon. The HycA protein acts as an antiactivator of FhlA Skibinski DA,20021HypF activates hyf operon transcription only when it is expressed from a heterologous promoter Skibinski DA,2002. Self WT,2004.1CRP and anaerobiosis are required for the hyf operon expression when the HyfR protein is the activator Self WT,2004.
2Only FhlA mutants can activate the transcription of the hyf operon. The HycA protein acts as an antiactivator of FhlA Skibinski DA,2002
3HypF activates hyf operon transcription only when it is expressed from a heterologous promoter Skibinski DA,2002. Self WT,2004.
4It seems that Fnr repress the hyf operon expression when it is activated by HyfR. Its is possible that binding of FNR may minimize the binding of HyfR, whose binding site is only 25 bases upstream of the FNR-binding site Self WT,2004.





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