RegulonDB RegulonDB 10.8: Operon Form
   

artPIQM operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: artPIQM
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: artPIQM
Gene(s): artM, artQ, artI, artP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Wissenbach U., et al., 1993
[2] Wissenbach U., et al., 1995
Promoter
Name: artPp
+1: 903769
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 35
Sequence: ttttaacttttaaagcagaaatattgcataattattctgtcaaaggtactatcttcgaccTcaatagctatcagactgcca
                        -35                    -10          +1                   
Note(s): Inhibition of artPp by ArgR is mediated by steric exclusion of the RNA polymerase Caldara M,2007
Evidence: [HIPP]
[TIM]
Reference(s): [3] Caldara M., et al., 2007
[2] Wissenbach U., et al., 1995
Terminator(s)
Type: rho-dependent
Sequence: tgaaataaaaAAGACGGACAACTTAGTGGGTTGTCCGTCTTcattataaga
Reference(s): [2] Wissenbach U., et al., 1995
TF binding sites (TFBSs)
[ArgR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArgR-L-arginine repressor artPp 903790 903808 -29.5 aaagcagaaaTATTGCATAATTATTCTGTcaaaggtact nd [AIBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(CHIP-SV/SM)], [CV(GEA/ROMA)], [CV(GEA/ROMA/SM)], [GEA], [SM] [3], [4], [5]
proximal ArgR-L-arginine repressor artPp 903811 903829 -50.5 tgcgctttgcTTTTAACTTTTAAAGCAGAaatattgcat nd [BPP], [CV(CHIP-SV/SM)], [GEA], [SM] [3]


Transcription unit       
Name: artPIQM
Gene(s): artM, artQ, artI, artP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [2] Wissenbach U., et al., 1995
Promoter
Name: artPp1
+1: 903815
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 81
Sequence: ccgcccccgttattttgtgctatgtttattgaataatgcgctttgcttttaacttttaaaGcagaaatattgcataattat
                             -35              -10           +1                   
Evidence: [TIM]
Reference(s): [6] Lacour S., et al., 2004
Terminator(s)
Type: rho-dependent
Sequence: tgaaataaaaAAGACGGACAACTTAGTGGGTTGTCCGTCTTcattataaga
Reference(s): [2] Wissenbach U., et al., 1995


Transcription unit       
Name: artPIQM
Gene(s): artM, artQ, artI, artP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [2] Wissenbach U., et al., 1995
Promoter
Name: artPp2
+1: 903836
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 102
Sequence: gacatttatgctcgccgaccaccgcccccgttattttgtgctatgtttattgaataatgcGctttgcttttaacttttaaa
                           -35             -10              +1                   
Evidence: [TIM]
Reference(s): [6] Lacour S., et al., 2004
Terminator(s)
Type: rho-dependent
Sequence: tgaaataaaaAAGACGGACAACTTAGTGGGTTGTCCGTCTTcattataaga
Reference(s): [2] Wissenbach U., et al., 1995


Transcription unit       
Name: artPIQM
Gene(s): artM, artQ, artI, artP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [2] Wissenbach U., et al., 1995
Promoter
Name: artPp3
+1: 903843
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 109
Sequence: gaatgccgacatttatgctcgccgaccaccgcccccgttattttgtgctatgtttattgaAtaatgcgctttgcttttaac
                          -35               -10             +1                   
Evidence: [TIM]
Reference(s): [6] Lacour S., et al., 2004
Terminator(s)
Type: rho-dependent
Sequence: tgaaataaaaAAGACGGACAACTTAGTGGGTTGTCCGTCTTcattataaga
Reference(s): [2] Wissenbach U., et al., 1995


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(CHIP-SV/SM)] cross validation(CHIP-SV/SM)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [CV(GEA/ROMA/SM)] cross validation(GEA/ROMA/SM)

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Wissenbach U., Keck B., Unden G., 1993, Physical map location of the new artPIQMJ genes of Escherichia coli, encoding a periplasmic arginine transport system., J Bacteriol 175(11):3687-8

 [2] Wissenbach U., Six S., Bongaerts J., Ternes D., Steinwachs S., Unden G., 1995, A third periplasmic transport system for L-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport., Mol Microbiol 17(4):675-86

 [3] Caldara M., Minh PN., Bostoen S., Massant J., Charlier D., 2007, ArgR-dependent repression of arginine and histidine transport genes in Escherichia coli K-12., J Mol Biol 373(2):251-67

 [4] Caldara M., Charlier D., Cunin R., 2006, The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation., Microbiology 152(Pt 11):3343-54

 [5] Makarova KS., Mironov AA., Gelfand MS., 2001, Conservation of the binding site for the arginine repressor in all bacterial lineages., Genome Biol 2(4):RESEARCH0013

 [6] Lacour S., Landini P., 2004, SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences., J Bacteriol 186(21):7186-95

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