RegulonDB RegulonDB 10.8: Operon Form
   

dhaKLM operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dhaKLM
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dhaKLM
Gene(s): dhaM, dhaL, dhaK   Genome Browser M3D Gene expression COLOMBOS
Note(s): The dhaKLM operon is induced by Dha. With this inductor, DhaL is dephosphorylated and DhaL::ADP (nonphosphorylated form) displaces DhaK and stimulates DhaR activity. In the absence of Dha, DhaL::ADP (nonphosphorylated form) is converted by PTS to DhaL::ATP (phosphorylated form), which does not bind to DhaR. DhaL functions as a coactivator and DhaK as a corepressor of dhaR transcription, respectively Bachler C,2005.
dhaKLM were found to be downregulated in a MG1655 lysogen carrying the Stx2a phage PA8 1208335|.
Evidence: [ITC] Inferred through co-regulation
Reference(s): [1] Bachler C., et al., 2005
Promoter
Name: dhaKp
+1: 1250944
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 106
Sequence: aaagtttcaatatggaacattcattaaagcatatgtttcactatgaaacataatttgggcTaattttcttttcccttgcct
                    -35              -10                    +1                   
Evidence: [AIPP]
Reference(s): [2] Mendoza-Vargas A., et al., 2009
TF binding sites (TFBSs)
[DhaR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DhaR1 activator dhaKp nd nd nd nd nd [GEA] [1]
Note(s): 1Between the dhaKLM operon and the dhaR gene there is a 190-bp-long intergenic region that divergently transcribes them. DhaR is an (auto)repressor of the dhaR gene and an activator of the dhaKLM operon Bachler C,2005.1Between the dhaKLM operon and the dhaR gene there is a 190-bp-long intergenic region that divergently transcribes them. DhaR is an (auto)repressor of the dhaR gene and an activator of the dhaKLM operon Bachler C,2005.


Transcription unit       
Name: dhaM
Gene(s): dhaM   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: dhaMp
+1: Unknown
Evidence: [IEP]
Reference(s): [3] Zaslaver A., et al., 2006


Evidence    

 [AIPP] Automated inference of promoter position

 [GEA] Gene expression analysis

 [IEP] Inferred from expression pattern


Reference(s)    

 [1] Bachler C., Schneider P., Bahler P., Lustig A., Erni B., 2005, Escherichia coli dihydroxyacetone kinase controls gene expression by binding to transcription factor DhaR., EMBO J 24(2):283-93

 [2] Mendoza-Vargas A., Olvera L., Olvera M., Grande R., Vega-Alvarado L., Taboada B., Jimenez-Jacinto V., Salgado H., Juarez K., Contreras-Moreira B., Huerta AM., Collado-Vides J., Morett E., 2009, Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli., PLoS One 4(10):e7526

 [3] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8

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