RegulonDB RegulonDB 10.8: Operon Form
   

hcaEFCBD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hcaEFCBD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: hcaEFCBD
Gene(s): hcaE, hcaF, hcaC, hcaB, hcaD   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[ITC] Inferred through co-regulation
Reference(s): [1] Turlin E., et al., 2005
Promoter
Name: hcaEp
+1: 2668982
Distance from start of the gene: 50
Sequence: cgtctcagctggtagaaattaactatttcacatattagcaaccaaccagcaacatccttaTggcacaaaaatagaaggtca
Note(s): The hcaEp promoter has a weak -10 region (CAACAT) and a good -35 region (TTCACA) Turlin E,2005.
Evidence: [TIM]
Reference(s): [1] Turlin E., et al., 2005
TF binding sites (TFBSs)
[HcaR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal HcaR-HCA activator hcaEp 2668934 2668946 -42.0 tctcagctggTAGAAATTAACTAtttcacatat nd [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2], [3]


Evidence    

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Turlin E., Sismeiro O., Le Caer JP., Labas V., Danchin A., Biville F., 2005, 3-phenylpropionate catabolism and the Escherichia coli oxidative stress response., Res Microbiol 156(3):312-21

 [2] Diaz E., Ferrandez A., Garcia JL., 1998, Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12., J Bacteriol 180(11):2915-23

 [3] Turlin E., Perrotte-piquemal M., Danchin A., Biville F., 2001, Regulation of the early steps of 3-phenylpropionate catabolism in Escherichia coli., J Mol Microbiol Biotechnol 3(1):127-33

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