RegulonDB RegulonDB 10.8: Operon Form
   

evgAS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: evgAS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: evgAS
Gene(s): evgA, evgS   Genome Browser M3D Gene expression COLOMBOS
Note(s): The evgAS operon was inferred based on the facts that evgA and evgS are adjacent genes, encoded in the same direction, and their products are part of the two-component system EvgS/EvgA. EvgS is the sensor protein and EvgA is theresponse regulator 8125343.
The expression of the gene evgA is increased under acidic growth conditions in either aerobiosis or microaerobiosis Marzan LW,2013 The increased expression under aerobiosis appears to be caused by the transcription factor PhoB Marzan LW,2013 but it is not known which promoter, of two transcribing evgA, is affected by PhoB.
Evidence: [IC] Inferred by curator
Promoter
Name: evgAp2
+1: 2483631
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 124
Sequence: atcggtacaatccctgattttattgttgacatttcatttatgccgactatttatatggtaTacttgtcgaattatcttaaa
                                 -35                  -10   +1                   
Evidence: [TIM]
Reference(s): [1] Tanabe H., et al., 1998
TF binding sites (TFBSs)
[EvgA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote EvgA-Phosphorylated activator evgAp2 2483513 2483530 -109.5 tgcaatacaaTTCTTACGCCTGTAGGATtagtaagaag nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2], [3]
[H-NS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor evgAp2 nd nd nd nd nd [BPP] [4]


Transcription unit          
Name: evgAS
Gene(s): evgA, evgS   Genome Browser M3D Gene expression COLOMBOS
Note(s): The evgAS operon was inferred based on the facts that evgA and evgS are adjacent genes, encoded in the same direction, and their products are part of the two-component system EvgS/EvgA. EvgS is the sensor protein and EvgA is theresponse regulator 8125343.
The expression of the gene evgA is increased under acidic growth conditions in either aerobiosis or microaerobiosis Marzan LW,2013 The increased expression under aerobiosis appears to be caused by the transcription factor PhoB Marzan LW,2013 but it is not known which promoter, of two transcribing evgA, is affected by PhoB.
Evidence: [IC] Inferred by curator
Promoter
Name: evgAp1
+1: 2483641
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 114
Sequence: tccctgattttattgttgacatttcatttatgccgactatttatatggtatacttgtcgaAttatcttaaaggaagctcag
                           -35              -10             +1                   
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [5] Salgado H, et al., 2012
[1] Tanabe H., et al., 1998
TF binding sites (TFBSs)
[EvgA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote EvgA-Phosphorylated activator evgAp1 2483513 2483530 -119.5 tgcaatacaaTTCTTACGCCTGTAGGATtagtaagaag nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2], [3]
[H-NS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor evgAp1 nd nd nd nd nd [BPP] [4]


Evidence    

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)

 [CV(TIM)] cross validation(TIM)

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Tanabe H., Yamasaki K., Katoh A., Yoshioka S., Utsumi R., 1998, Identification of the promoter region and the transcriptional regulatory sequence of the evgAS operon of Escherichia coli., Biosci Biotechnol Biochem 62(2):286-90

 [2] Eguchi Y., Oshima T., Mori H., Aono R., Yamamoto K., Ishihama A., Utsumi R., 2003, Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli., Microbiology 149(Pt 10):2819-28

 [3] Masuda N., Church GM., 2003, Regulatory network of acid resistance genes in Escherichia coli., Mol Microbiol 48(3):699-712

 [4] Krin E., Danchin A., Soutourina O., 2010, Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coli., BMC Microbiol 10:273

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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