RegulonDB RegulonDB 10.10: Operon Form
   

ftsK operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: ftsK
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: ftsK
Gene(s): ftsK   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: ftsKp2
+1: 932929
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 295
Sequence: aattcaataccgctgtacaaaaacttgaagaaattcaggagtgtcatttagtatccggtgAtttcgactacctgttgaaaa
                         -35                     -10        +1                   
Note(s): ftsKp2 is a nonessential promoter and it does not transcribe ftsK up to significant levels, except in the presence of ftsKp1 Dorazi R,2000
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [1] Dorazi R., et al., 2000
[2] Huerta AM., et al., 2003


Transcription unit          
Name: ftsK
Gene(s): ftsK   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: ftsKp1
+1: 933138
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 86
Sequence: aagacgcgctaacacggaacaggtgcaaaatcggcgtattttgattacactcctgttaatCcatacagcaacagtactggg
                       -35                    -10           +1                   
Note(s): ftsKp1 (dinH) is the main ftsK promoter during the cellular division process. Dorazi R,2000
Although a primer extension experiment has been done for this operon, the results indicated a region
(2-4 nucleotides) rather than a specific start site Dorazi R,2000 We have decided to place the transcription +1 site in the nucleotide closest to the -10 box.
Evidence: [AIPP]
[ICWHO]
[TIM]
Reference(s): [1] Dorazi R., et al., 2000
[2] Huerta AM., et al., 2003
[3] Lewis LK., et al., 1992
TF binding sites (TFBSs)
[LexA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor ftsKp1 933128 933147 -0.5 ttgattacacTCCTGTTAATCCATACAGCAacagtactgg nd [AIBSCS], [GEA], [AIBSCS], [BPP] [1], [3]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp activation ftsKp1 nd   [GEA] [4]
Evidence: [GEA] Gene expression analysis
Reference(s): [4] Diez A., et al., 2000


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [AIPP] Automated inference of promoter position

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Dorazi R., Dewar SJ., 2000, The SOS promoter dinH is essential for ftsK transcription during cell division., Microbiology 146 ( Pt 11):2891-9

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Lewis LK., Jenkins ME., Mount DW., 1992, Isolation of DNA damage-inducible promoters in Escherichia coli: regulation of polB (dinA), dinG, and dinH by LexA repressor., J Bacteriol 174(10):3377-85

 [4] Diez A., Gustavsson N., Nystrom T., 2000, The universal stress protein A of Escherichia coli is required for resistance to DNA damaging agents and is regulated by a RecA/FtsK-dependent regulatory pathway., Mol Microbiol 36(6):1494-503

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