RegulonDB RegulonDB 10.7: Operon Form
   

prpBCDE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: prpBCDE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: prpBCDE
Gene(s): prpB, prpC, prpD, prpE   Genome Browser M3D Gene expression COLOMBOS
Note(s): AscG may interfere with the activation of the prpBCDE operon by PrpR |CITS:[19633077]|.
A new carbon catabolite repression (CCR) system was determined between carbohydrate-based carbon sources (sugars) and noncarbohydrate-based carbon sources (e.g., propionate).Expression of the prpBCDE operon is repressed in the presence of various sugars (arabinose, galactose, glucose, mannose, and xylose), and this repression is independent of sugar-specific regulators, such as AraC, cAMP-CRP, and the phosphotransferase system (PTS), during rapid sugar uptake and metabolism |CITS:[22579471]|.
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: prpBp
+1: 348646
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 36
Sequence: tgaaattaaacatttaattttattaaggcaattgtggcacaccccttgctttgtctttatCaacgcaaataacaagttgat
                                   -24         -12          +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Evidence: [AIPP]
Reference(s): [1] Reitzer L., et al., 2001
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator prpBp 348529 348551 -106.5 acattgcctgAAACGTTAACTGAAACGCATATTtgcggattag nd [APIORCISFBSCS], [BPP], [GEA], [SM] [2], [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Cra repressor prpBp 348536 348553 -101.5 ctgaaacgttAACTGAAACGCATATTTGcggattagtt nd [AIBSCS], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote PrpR-(2S,3S)-2-methylcitrate activator prpBp 348487 348510 -147.5 gtttagtcttGTTTCATAATTGTTGCAATGAAACgcggtgaaac nd [APIORCISFBSCS], [GEA] [2]
remote PrpR-(2S,3S)-2-methylcitrate activator prpBp 348521 348544 -113.5 gcggtgaaacATTGCCTGAAACGTTAACTGAAACgcatatttgc nd [APIORCISFBSCS] [2]





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