RegulonDB RegulonDB 11.0: Operon Form
   

fliE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fliE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fliE
Gene(s): fliE   Genome Browser M3D Gene expression COLOMBOS
Note(s): In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of fliE as well as other flagellar genes Li Y, Liu B, Guo J, Cong H, He S, Zhou H, Zhu F, Wang Q, Zhang L,2019.
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Reference(s): [1] Stafford GP., et al., 2005
Promoter
Name: fliEp1
+1: 2013036
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 22
Sequence: cgtttttttgttgctatttagcgcctttgtcttattgacttactggtaggctttgctaccAgaaattatccgggagacgag
                          -35                  -10          +1                   
Note(s): We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, are present at a distance of 6 nucleotides between the transcriptional start site and the -10 box.
In a cell-free expression system, FlhDC slightly activated fliE expression while σ28 did not show an effect on fliE expression Maddalena LL, Niederholtmeyer H, Turtola M, Swank ZN, Belogurov GA, Maerkl SJ,2016
Evidence: [HIPP]
[ICWHO]
[IDA]
[IHBCE]
Reference(s): [2] Brandi A., et al., 2016
[3] Huerta AM., et al., 2003
[1] Stafford GP., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CsgD repressor fliEp1 2013054 2013068 -24.5 tagcgcctttGTCTTATTGACTTACtggtaggctt nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP] [5]
proximal CsgD repressor fliEp1 2013069 2013083 -39.5 ttttttgttgCTATTTAGCGCCTTTgtcttattga nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator fliEp1 2013068 2013086 -40.5 cgtttttttgTTGCTATTTAGCGCCTTTGtcttattgac nd [GEA], [APIORCISFBSCS] [4]
proximal FlhDC activator fliEp1 2013097 2013113 -69.0 tcgtttttttCTATCCGCCAATAAACCcgtttttttg nd [GEA], [APIORCISFBSCS] [4]


Transcription unit          
Name: fliE
Gene(s): fliE   Genome Browser M3D Gene expression COLOMBOS
Note(s): In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flgBCDEFGHIJ as well as other flagellar genes Li Y, Liu B, Guo J, Cong H, He S, Zhou H, Zhu F, Wang Q, Zhang L,2019.
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
[BTEI] Boundaries of transcription experimentally identified
Reference(s): [6] Muller V., et al., 1992
Promoter
Name: fliEp2
+1: 2013093
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 79
Sequence: gaaaaattgacgctcccattatttgtgctttcgtttttttctatccgccaataaacccgtTtttttgttgctatttagcgc
                          -35                  -10          +1                   
Evidence: [APIORCISFBSCS]
[HIPP]
[ICWHO]
Reference(s): [3] Huerta AM., et al., 2003
[6] Muller V., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CsgD repressor fliEp2 2013054 2013068 33.5 tagcgcctttGTCTTATTGACTTACtggtaggctt nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP] [5]
proximal CsgD repressor fliEp2 2013069 2013083 18.5 ttttttgttgCTATTTAGCGCCTTTgtcttattga nd [APIORCISFBSCS], [BPP], [GEA], [APIORCISFBSCS], [BPP] [5]





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