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| Operon |  |
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| Name: | cpxRA |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | cpxRA | |||||||||
| Gene(s): | cpxA, cpxR Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | The cpxRA operon was inferred based on the facts that cpxR and cpxA are adjacent genes, encoded in the same direction, and their products are part of the two-component system CpxR/CpxA. CpxA is the sensor protein and CpxR is the response regulator De Wulf P,1999. An integrated transcriptomic study of the extracytoplasmic stress response, which detects and responds to alterations of the bacterial envelope, was performed by Bury-Moré et al. (2009). They investigated the interconnections and dynamics between Bae, Cpx, Psp, Rcs, and σE pathways and showed that these pathways can be activated simultaneously in response to exogenous or endogenous stimulation, while natural environmental stimuli provoke bacterial modifications that lead to multiple pathway responses Bury-Moné S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P,2009 Each of these responses might be specialized to ensure a specific aspect of envelope biogenesis and maintenance; on the other hand, CpxR could have a role as a modulator of the response by integrating other endogenous signals. Bury-Moré et al. concluded that all five pathways are needed to mount a full response to extracytoplasmic stress Bury-Moné S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P,2009 |
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| Evidence: | [IC] Inferred by curator | |||||||||
| Promoter | ||||||||||
| Name: | cpxRp2 | |||||||||
| +1: | 4105686 | |||||||||
| Sigma Factor: | Sigma38 Sigmulon | |||||||||
| Distance from start of the gene: | 16 | |||||||||
| Sequence: |
gggggtgtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctCggaggtatttaaacaatgaa |
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| Note(s): | De Wulf et al. in 1999 showed that transcription of cpxR is highly dependent on alternative factor σ38 (RpoS) during the stationary phase De Wulf P,1999. We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box. |
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| Evidence: |
[HIPP] [IEP] |
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| Reference(s): |
[1] De Wulf P., et al., 1999 [2] Dudin O., et al., 2014 |
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| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CpxR-phosphorylated | activator | cpxRp2 | 4105726 | 4105740 | -47.0 | gtcagggggtGTAAAACAACGTAAAgtcatggatt | nd | [GEA], [APIORCISFBSCS], [BPP] | [1], [4] |
| proximal | CpxR-phosphorylated | activator | cpxRp2 | 4105746 | 4105760 | -67.0 | gattcaggctGCAAACATGCGTCAGggggtgtaaa | nd | [GEA], [APIORCISFBSCS] | [1], [4] |
| Name: | cpxRA | |||||||||
| Gene(s): | cpxA, cpxR Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | The cpxRA operon was inferred based on the facts that cpxR and cpxA are adjacent genes, encoded in the same direction, and their products are part of the two-component system CpxR/CpxA. CpxA is the sensor protein and CpxR is the response regulator De Wulf P,1999. An integrated transcriptomic study of the extracytoplasmic stress response, which detects and responds to alterations of the bacterial envelope, was performed by Bury-Moré et al. (2009). They investigated the interconnections and dynamics between Bae, Cpx, Psp, Rcs, and σE pathways and showed that these pathways can be activated simultaneously in response to exogenous or endogenous stimulation, while natural environmental stimuli provoke bacterial modifications that lead to multiple pathway responses Bury-Moné S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P,2009 Each of these responses might be specialized to ensure a specific aspect of envelope biogenesis and maintenance; on the other hand, CpxR could have a role as a modulator of the response by integrating other endogenous signals. Bury-Moré et al. concluded that all five pathways are needed to mount a full response to extracytoplasmic stress Bury-Moné S, Nomane Y, Reymond N, Barbet R, Jacquet E, Imbeaud S, Jacq A, Bouloc P,2009 |
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| Evidence: | [IC] Inferred by curator | |||||||||
| Promoter | ||||||||||
| Name: | cpxRp | |||||||||
| +1: | 4105686 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 16 | |||||||||
| Sequence: |
gggggtgtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctCggaggtatttaaacaatgaa -35 -10 +1 |
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| Note(s): | De Wulf et al. in 1999 showed that transcription of cpxR is highly dependent on alternative factor σ38 (RpoS) during the stationary phase De Wulf P,1999. We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box. |
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| Evidence: |
[HIPP] [ICWHO] |
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| Reference(s): |
[1] De Wulf P., et al., 1999 [5] Huerta AM., et al., 2003 |
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| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CpxR-phosphorylated | activator | cpxRp | 4105726 | 4105740 | -47.0 | gtcagggggtGTAAAACAACGTAAAgtcatggatt | nd | [GEA], [APIORCISFBSCS], [BPP] | [1], [4] |
| proximal | CpxR-phosphorylated | activator | cpxRp | 4105746 | 4105760 | -67.0 | gattcaggctGCAAACATGCGTCAGggggtgtaaa | nd | [GEA], [APIORCISFBSCS] | [1], [4] |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position