RegulonDB RegulonDB 10.8: Operon Form
   

spy operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: spy
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: spy
Gene(s): spy   Genome Browser M3D Gene expression COLOMBOS
Note(s): spy gene expression is induced separately by both copper and zinc metals through the CpxAR and BaeSR sensor response regulatory systems, respectively. BaeR can be the primary regulator and CpxR can enhance its function Yamamoto K,2008. 24999585 On the other hand, BaeR is the primary regulator of spy in the presence of the protein-unfolding agent ethanol 24999585
The expression of the gene spy is increased when colicin (at 30 ng/ml) is added to the medium 23421615. spy is also induced by indole Hirakawa H,2005. Raffa RG,2002.
Under conditions leading to NlpE outer membrane lipoprotein overexpression, the transcription of the spy gene is increased by CpxR Raivio TL,2013
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Hagenmaier S., et al., 1997
[2] Nishino K., et al., 2005
Promoter
Name: spyp
+1: 1825688
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 63
Sequence: acactttcattgttttaccgttgctctgattaattgacgctaaagtcagtaaagttaatcTcgtcaacacggcacgctact
                             -35                  -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Hagenmaier S., et al., 1997
[3] Yamamoto K., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: ttgagaagagAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTtttggctgaa
Reference(s): [1] Hagenmaier S., et al., 1997
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal BaeR-Phosphorylated activator spyp 1825765 1825784 -86.5 atcaaattttCTTTTTTTCTCCATAATTGGcgcaaaagtg nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [4], [5]
remote BaeR-Phosphorylated activator spyp 1825836 1825855 -157.5 ccagtcatccGGTATAGTTCTTCATAATCTctgcaaaatc nd [APIORCISFBSCS], [BPP], [IC] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CpxR-Phosphorylated1 activator spyp 1825731 1825745 -50.0 gttttttacaCTTTCATTGTTTTACcgttgctctg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [3], [5], [6], [7]
Note(s): 1The consensus of this CpxR-binding site is not located in the same DNA strand as in the spyp promoter Yamamoto K,20063The consensus of this CpxR-binding site is not located in the same DNA strand as in the spyp promoter Yamamoto K,2006





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