RegulonDB RegulonDB 10.8: Operon Form

flgBCDEFGHIJ operon and associated TUs in Escherichia coli K-12 genome

This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Gene(s): flgB, flgC, flgD, flgE, flgF, flgG, flgH, flgI, flgJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): flgBCDEFGHIJ expression is enhanced by AtoSC in the presence of acetoacetate 22083893.
In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flgBCDEFGHIJ as well as other flagellar genes 31271063.
Reference(s): [1] Fahrner KA., et al., 1994
[2] Jones CJ., et al., 1987
[3] Macnab RM. 1992
Name: flgBp
+1: 1130993
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: gacccattttgcgtttattccgccgataacgcgcgcgtaaaggcatttaagctgatggcaGaattttgatacctgcggagg
                          -35                   -10         +1                   
Note(s): We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, present a distance of 6 nucleotides between the transcriptional start site and the -10 box.
Evidence: [HIPP]
Reference(s): [4] Stafford GP., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator flgBp 1130917 1130933 -67.5 caatgaccatCAACGGCATAAATAGCGacccattttg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [4], [5]
proximal FlhDC activator flgBp 1130943 1130959 -41.5 gacccattttGCGTTTATTCCGCCGATaacgcgcgcg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [4]

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -12.2 1135511 1135551 gcaactctaaGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGaagatgatag
  anti-terminator -6.2 1135488 1135528 gatcagatgcTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGgttcaccggt
  anti-anti-terminator -10.2 1135457 1135499 cgaaatcaacAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGacgcaactct
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"