RegulonDB RegulonDB 11.1: Operon Form
   

flgBCDEFGHIJ operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: flgBCDEFGHIJ
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: flgBCDEFGHIJ
Gene(s): flgB, flgC, flgD, flgE, flgF, flgG, flgH, flgI, flgJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): flgBCDEFGHIJ expression is enhanced by AtoSC in the presence of acetoacetate Theodorou MC, Theodorou EC, Kyriakidis DA,2012.
In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flgBCDEFGHIJ as well as other flagellar genes Li Y, Liu B, Guo J, Cong H, He S, Zhou H, Zhu F, Wang Q, Zhang L,2019.
Reference(s): [1] Fahrner KA., et al., 1994
[2] Jones CJ., et al., 1987
[3] Macnab RM. 1992
Promoter
Name: flgBp
+1: 1130993
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: gacccattttgcgtttattccgccgataacgcgcgcgtaaaggcatttaagctgatggcaGaattttgatacctgcggagg
                          -35                   -10         +1                   
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA]
Reference(s): [4] Brandi A., et al., 2016
[5] Huerta AM., et al., 2003
[6] Stafford GP., et al., 2005
TF binding sites (TFBSs)
[FlhDC,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator flgBp 1130917 1130933 -67.5 caatgaccatCAACGGCATAAATAGCGacccattttg nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [6]
proximal FlhDC activator flgBp 1130943 1130959 -41.5 gacccattttGCGTTTATTCCGCCGATaacgcgcgcg nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [6]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -12.2 1135511 1135551 gcaactctaaGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGaagatgatag
  anti-terminator -6.2 1135488 1135528 gatcagatgcTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGgttcaccggt
  anti-anti-terminator -10.2 1135457 1135499 cgaaatcaacAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGacgcaactct
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA] Inferred from direct assay

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Fahrner KA., Block SM., Krishnaswamy S., Parkinson JS., Berg HC., 1994, A mutant hook-associated protein (HAP3) facilitates torsionally induced transformations of the flagellar filament of Escherichia coli., J Mol Biol 238(2):173-86

 [2] Jones CJ., Homma M., Macnab RM., 1987, Identification of proteins of the outer (L and P) rings of the flagellar basal body of Escherichia coli., J Bacteriol 169(4):1489-92

 [3] Macnab RM., 1992, Genetics and biogenesis of bacterial flagella., Annu Rev Genet 26:131-58

 [4] Brandi A., Giangrossi M., Giuliodori AM., Falconi M., 2016, An Interplay among FIS, H-NS, and Guanosine Tetraphosphate Modulates Transcription of the Escherichia coli cspA Gene under Physiological Growth Conditions., Front Mol Biosci 3:19

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Stafford GP., Ogi T., Hughes C., 2005, Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2., Microbiology 151(Pt 6):1779-88

RegulonDB