RegulonDB RegulonDB 10.8: Operon Form
   

flhBAE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: flhBAE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: flhBAE
Gene(s): flhE, flhA, flhB   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a consensus sequence for a σ28 factor at 120 bp upstream of the transcription initiation site of the flhBp promoter, which could be a second promoter for the flhB operon Liu X,1994 The DNA sequence was not shown.
In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flhB as well as other flagellar genes 31271063.
Evidence: [ITC] Inferred through co-regulation
Promoter
Name: flhBp
+1: 1966216
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: cgcctgttttgccccttactcaaaccattgaacgctttgcgctctggcatcattcacgctTaatactctttccaggattgg
Evidence: [HIPP]
[TAS]
Reference(s): [1] Liu X., et al., 1994
TF binding sites (TFBSs)
[FlhDC,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator flhBp 1966247 1966263 -39.0 ctgttttgccCCTTACTCAAACCATTGaacgctttgc nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2]
proximal FlhDC activator flhBp 1966275 1966293 -67.5 atgtgtggcaGCAAAAGCCCTAAATCCCGcctgttttgc nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [1], [2]


Evidence    

 [HIPP] Human inference of promoter position

 [TAS] Traceable author statement

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Liu X., Matsumura P., 1994, The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons., J Bacteriol 176(23):7345-51

 [2] Stafford GP., Ogi T., Hughes C., 2005, Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2., Microbiology 151(Pt 6):1779-88

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