RegulonDB RegulonDB 11.2: Operon Form
   

flhBAE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: flhBAE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: flhBAE
Gene(s): flhE, flhA, flhB   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a consensus sequence for a σ28 factor at 120 bp upstream of the transcription initiation site of the flhBp promoter, which could be a second promoter for the flhB operon Liu X,1994 The DNA sequence was not shown.
In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flhB as well as other flagellar genes Li Y, Liu B, Guo J, Cong H, He S, Zhou H, Zhu F, Wang Q, Zhang L,2019.
Evidence: [EXP-IEP-COREGULATION] Inferred through co-regulation
Promoter
Name: flhBp
+1: 1966216
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: cgcctgttttgccccttactcaaaccattgaacgctttgcgctctggcatcattcacgctTaatactctttccaggattgg
Evidence: [AS-TAS]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA]
Reference(s): [1] Liu X., et al., 1994
[2] Liu X., et al., 1996
TF binding sites (TFBSs)
[FlhDC,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator flhBp 1966247 1966263 -39.0 ctgttttgccCCTTACTCAAACCATTGaacgctttgc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] C [1], [3]
proximal FlhDC activator flhBp 1966275 1966293 -67.5 atgtgtggcaGCAAAAGCCCTAAATCCCGcctgttttgc nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-SEQ], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] C [1], [3]


Evidence    

 [AS-TAS] Traceable author statement

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA] Inferred from direct assay

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-CHIP-SEQ] high throughput ChIP-seq evidence

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Liu X., Matsumura P., 1994, The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons., J Bacteriol 176(23):7345-51

 [2] Liu X., Matsumura P., 1996, Differential regulation of multiple overlapping promoters in flagellar class II operons in Escherichia coli., Mol Microbiol 21(3):613-20

 [3] Stafford GP., Ogi T., Hughes C., 2005, Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2., Microbiology 151(Pt 6):1779-88

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