RegulonDB RegulonDB 10.7: Operon Form
   

pgaABCD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pgaABCD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: pgaABCD
Gene(s): pgaD, pgaC, pgaB, pgaA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The pgABCD operon was inferred based on the facts that its genes are adjacent, encoded in the same direction, and their products are all involved in biofilm formation |CITS:[15090514]|.
H-NS represses pgaA transcription, although it is unknown if the effect is direct or indirect |CITS:[28470798]|.
Evidence: [IC] Inferred by curator
Promoter
Name: pgaAp
+1: 1092523
Distance from start of the gene: 234
Sequence: ccgaaatcatgcatcggaatttactgatttaattattttaatcctaatttattttgaaaaAggcattgggatttatgccgt
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [1] Goller C., et al., 2006
[2] Wang X., et al., 2005
TF binding sites (TFBSs)
[NhaR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NhaR-Na+ activator pgaAp 1092556 1092572 -41.0 cgaaatcatgCATCGGAATTTACTGATttaattattt nd [APIORCISFBSCS], [BPP], [GEA] [1], [3]
proximal NhaR-Na+ activator pgaAp 1092578 1092594 -63.0 tcatcaggacTTTCGAAAAATCCGAAAtcatgcatcg nd [APIORCISFBSCS], [BPP], [GEA] [1], [3]
[OmpR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OmpR-P1 repressor pgaAp 1092545 1092564 -31.5 tgcatcggaaTTTACTGATTTAATTATTTTaatcctaatt nd [APIORCISFBSCS], [BPP], [GEA] [4]
Note(s): 1OmpR-P represses biofilm production by direct interaction of the pgaABCD operon through acetyl phosphate as the phosphodonor Oropeza R,20153OmpR-P represses biofilm production by direct interaction of the pgaABCD operon through acetyl phosphate as the phosphodonor Oropeza R,2015


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  mcaS            
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Goller C., Wang X., Itoh Y., Romeo T., 2006, The cation-responsive protein NhaR of Escherichia coli activates pgaABCD transcription, required for production of the biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine., J Bacteriol 188(23):8022-32

 [2] Wang X., Dubey AK., Suzuki K., Baker CS., Babitzke P., Romeo T., 2005, CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli., Mol Microbiol 56(6):1648-63

 [3] Cerca N., Jefferson KK., 2008, Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli., FEMS Microbiol Lett 283(1):36-41

 [4] Oropeza R., Salgado-Bravo R., Calva E., 2015, Deletion analysis of RcsC reveals a novel signalling pathway controlling poly-N-acetylglucosamine synthesis and biofilm formation in Escherichia coli., Microbiology 161(Pt 4):903-13

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