RegulonDB RegulonDB 11.1: Operon Form
   

pgaABCD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pgaABCD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: pgaABCD
Gene(s): pgaD, pgaC, pgaB, pgaA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The pgABCD operon was inferred based on the facts that its genes are adjacent, encoded in the same direction, and their products are all involved in biofilm formation Wang X, Preston JF, Romeo T,2004
H-NS represses pgaA transcription, although it is unknown if the effect is direct or indirect Parker A,2017
The Rho protein appears to negatively regulate expression of the operon pgaABCD during the log phase of growth Hafeezunnisa M, Sen R,2020.
Evidence: [IC] Inferred by curator
Promoter
Name: pgaAp
+1: 1092523
Distance from start of the gene: 234
Sequence: ccgaaatcatgcatcggaatttactgatttaattattttaatcctaatttattttgaaaaAggcattgggatttatgccgt
Evidence: [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Goller C., et al., 2006
[2] Wang X., et al., 2005
TF binding sites (TFBSs)
[NhaR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NhaR-Na+ activator pgaAp 1092556 1092572 -41.0 cgaaatcatgCATCGGAATTTACTGATttaattattt nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1], [3]
proximal NhaR-Na+ activator pgaAp 1092578 1092594 -63.0 tcatcaggacTTTCGAAAAATCCGAAAtcatgcatcg nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1], [3]
[OmpR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OmpR-phosphorylated repressor pgaAp 1092545 1092564 -31.5 tgcatcggaaTTTACTGATTTAATTATTTTaatcctaatt nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [4]


Evidence    

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Goller C., Wang X., Itoh Y., Romeo T., 2006, The cation-responsive protein NhaR of Escherichia coli activates pgaABCD transcription, required for production of the biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine., J Bacteriol 188(23):8022-32

 [2] Wang X., Dubey AK., Suzuki K., Baker CS., Babitzke P., Romeo T., 2005, CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli., Mol Microbiol 56(6):1648-63

 [3] Cerca N., Jefferson KK., 2008, Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli., FEMS Microbiol Lett 283(1):36-41

 [4] Oropeza R., Salgado-Bravo R., Calva E., 2015, Deletion analysis of RcsC reveals a novel signalling pathway controlling poly-N-acetylglucosamine synthesis and biofilm formation in Escherichia coli., Microbiology 161(Pt 4):903-13

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