RegulonDB RegulonDB 10.8: Operon Form
   

dsrA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dsrA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dsrA
Gene(s): dsrA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [AISDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: dsrAp
+1: 2025313
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 0
Sequence: ttaatcattcatatggcgaatattttcttgtcagcgaaaaaaattgcggataaggtgatgAacacatcagatttcctggtg
                            -35                    -10      +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Sledjeski D., et al., 1995
TF binding sites (TFBSs)
[LeuO,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd LeuO repressor dsrAp nd nd nd nd nd [GEA] [2]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  DksA-ppGpp activation dsrAp DksA and ppGpp activate the dsrA promoter, improving rpoS translation Girard ME,2018 On the other hand, DksA-ppGpp can increase dsrA expression indirectly by another mechanism, possibly by reducing rRNA transcription and thereby increasing RNAP availability Girard ME,2018 [APPH]
[GEA] 		[3]
Evidence: [APPH] Assay of protein purified to homogeneity
[GEA] Gene expression analysis
Reference(s): [3] Girard ME., et al., 2018


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis


Reference(s)    

 [1] Sledjeski D., Gottesman S., 1995, A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli., Proc Natl Acad Sci U S A 92(6):2003-7

 [2] Repoila F., Gottesman S., 2001, Signal transduction cascade for regulation of RpoS: temperature regulation of DsrA., J Bacteriol 183(13):4012-23

 [3] Girard ME., Gopalkrishnan S., Grace ED., Halliday JA., Gourse RL., Herman C., 2018, DksA and ppGpp Regulate the σS Stress Response by Activating Promoters for the Small RNA DsrA and the Anti-Adapter Protein IraP., J Bacteriol 200(2)

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