RegulonDB RegulonDB 10.10: Operon Form
   

fadE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fadE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fadE
Gene(s): fadE   Genome Browser M3D Gene expression COLOMBOS
Note(s): The fadE gene expression is enhanced when the fadD gene, which encodes the acyl-CoA synthetase, is overexpressed. The acyl-CoA probably binds to the FadR transcriptional regulator to inactivate it and avoids its repressive effect on fadE Zhang H, Wang P, Qi Q,2006
Evidence: [AISGDTU] Automated inference that a single-gene directon is a transcription unit
Promoter
Name: fadEp
+1: 243406
Distance from start of the gene: 103
Sequence: ggtgctatgaccaggactttttgacctgaagtgcggataaaaacagcaacaatgtgagctTtgttgtaattatattgtaaa
Evidence: [AIPP]
Reference(s): [1] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[ArcA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArcA-phosphorylated repressor fadEp 243385 243399 15.0 gctttgttgtAATTATATTGTAAACatattgctaa nd [EME], [GEA], [AIBSCS], [AIBSPD], [BPP], [CCE] [2], [3]
[FadR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote FadR repressor fadEp 243325 243341 74.0 atatcatcacAAGTGGTCAGACCTCCTacaagtaagg nd [GEA], [APIORCISFBSCS], [BPP] [4]


Evidence    

 [AIPP] Automated inference of promoter position

 [EME] Expression microarray evidence

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [AIBSPD] Automated inference based on sequence pattern discovery

 [BPP] Binding of purified proteins

 [CCE] ChIP-chip evidence

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Cho BK., Knight EM., Palsson BO., 2006, Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA., Microbiology 152(Pt 8):2207-19

 [3] Federowicz S., Kim D., Ebrahim A., Lerman J., Nagarajan H., Cho BK., Zengler K., Palsson B., 2014, Determining the control circuitry of redox metabolism at the genome-scale., PLoS Genet 10(4):e1004264

 [4] Campbell JW., Cronan JE., 2002, The enigmatic Escherichia coli fadE gene is yafH., J Bacteriol 184(13):3759-64

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