|Gene(s):||yecF Genome Browser M3D Gene expression COLOMBOS|
|Evidence:||[AISDTU] Automated inference that a single-gene directon is a transcription unit|
|Sigma Factor:||Sigma28 Sigmulon|
|Distance from start of the gene:||101|
-35 -10 +1
|Note(s):||Yu et al. showed in 2006 that transcription of yecF is highly dependent on alternative factor σF and can be transcribed by RNA polymerase σ28 in vitro Yu HH,2006.
The sequence of this promoter has been defined, by computational analyses, from the consensus sequence of the σ28 (σF) promoters: TAAAGTTT in the -35 box and GCCGATAA in the -10 box Yu HH,2006. Therefore, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box Yu HH, Di Russo EG, Rounds MA, Tan M,2006. Yu HH,2006. Park K,2001.
|Reference(s):|| Yu HH., et al., 2006|
 Feng CQ., et al., 2019
 Lesnik EA., et al., 2001