RegulonDB RegulonDB 10.10: Operon Form
   

kbaZ-agaVWA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: kbaZ-agaVWA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: kbaZ-agaVWA
Synonym(s): agaZVWA, kbaZagaVWEFA
Gene(s): kbaZ, agaV, agaW, agaA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ITCR] Inferred through co-regulation
Reference(s): [1] Brinkkotter A., et al., 2000
Promoter
Name: kbaZp
+1: 3278866
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 48
Sequence: agatcagaaaaccattatctttcgttttatttttatctcaccatgacgcagtatcaactgAaacaaaacgaaagattaata
                          -35                       -10     +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Ray WK., et al., 2004
TF binding sites (TFBSs)
[AgaR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal AgaR repressor kbaZp 3278781 3278800 -75.5 tttcagtgacTTTCATTATGTTTCTTTTGTgaatcagatc nd [GEA], [APIORCISFBSCS], [BPP] [3]
proximal AgaR repressor kbaZp 3278825 3278844 -31.5 aaccattatcTTTCGTTTTATTTTTATCTCaccatgacgc nd [GEA], [APIORCISFBSCS], [BPP] [3]
proximal AgaR repressor kbaZp 3278859 3278878 3.5 tgacgcagtaTCAACTGAAACAAAACGAAAgattaatatc nd [GEA], [APIORCISFBSCS], [BPP] [3]
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator kbaZp 3278795 3278816 -60.5 attatgtttcTTTTGTGAATCAGATCAGAAAAccattatctt nd [GEA], [APIORCISFBSCS], [BPP] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Brinkkotter A., Kloss H., Alpert C., Lengeler JW., 2000, Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli., Mol Microbiol 37(1):125-35

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Ray WK., Larson TJ., 2004, Application of AgaR repressor and dominant repressor variants for verification of a gene cluster involved in N-acetylgalactosamine metabolism in Escherichia coli K-12., Mol Microbiol 51(3):813-26

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