RegulonDB RegulonDB 10.6.3: Operon Form
   

xylE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: xylE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Gene(s): xylE   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on a combination of genetic, functional, and biochemical analyses, regulatory elements were identified in the regulatory region of xylE |CITS:[29728462]|.
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: xylEp
+1: 4242291
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 37
Sequence: gtaaacgcattgtaaaaaatgataattgccttaactgcctgacaattccaacatcaatgcActgataaaagatcagaatgg
Evidence: [AIBSPD]
[IC]
Reference(s): [1] Belliveau NM., et al., 2018
Terminator(s)
Type: rho-independent
Sequence: aagcgggagtAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGattacagcgt
Reference(s): [2] Davis EO., et al., 1987
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator xylEp 4242364 4242379 -80.0 gataattatcACAATTAAGATCACAGaaaagacatt nd [AIBSCS], [BPP], [GEA], [SM] [1]
[XylR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal XylR-xylose activator xylEp 4242327 4242343 -44.0 acgtaaacgcATTGTAAAAAATGATAAttgccttaac nd [AIBSCS], [BPP], [GEA], [SM] [1]
proximal XylR-xylose activator xylEp 4242348 4242364 -65.0 taagatcacaGAAAAGACATTACGTAAacgcattgta nd [AIBSCS], [BPP], [GEA], [SM] [1]


Evidence    

 [AIBSPD] Automated inference based on sequence pattern discovery

 [IC] Inferred by curator

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Belliveau NM., Barnes SL., Ireland WT., Jones DL., Sweredoski MJ., Moradian A., Hess S., Kinney JB., Phillips R., 2018, Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria., Proc Natl Acad Sci U S A 115(21):E4796-E4805

 [2] Davis EO., Henderson PJ., 1987, The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12., J Biol Chem 262(29):13928-32

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