RegulonDB RegulonDB 10.8: Operon Form
   

yhjX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: yhjX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: yhjX
Gene(s): yhjX   Genome Browser M3D Gene expression COLOMBOS
Note(s): Expression levels of cspI, ypjB, and yhjX genes were repressed by overproduction of YehT Kraxenberger T,2012 Insomuch as all of these genes lack any LytTR-like sequence motif, it is probable that the effect of YehT on these genes is indirect Kraxenberger T,2012
A functional link between the two systems provides positive feedback between YpdA/YhjX and yjiY transcription, while yhjX is negatively regulated by BtsS/YjiY activity Behr S,2014. 28796832
Based on genomic SELEX (gSELEX) screening it was demonstrated that in the presence of a high concentration of pyruvate, PyrSR regulates at least eight novel targets, including the yhjX gene, which encodes a protein with as-yet-unidentified function Miyake Y,2019
The yhjX gene is more highly expressed during exponential growth than stationary phase and it is the major target of PyrR Miyake Y,2019
Evidence: [ICWHO] Inferred computationally without human oversight
[LTED] Length of transcript experimentally determined
Reference(s): [1] Fried L., et al., 2013
Promoter
Name: yhjXp
+1: 3712043
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 36
Sequence: gatggcatttcatgccgtttttccccaggcataaagtgcacttcgttatggttgtcggcaGagatttttcctttttattac
                    -35                        -10          +1                   
Note(s): The transcription start site for yhjXp was determined by 5-RACE; however, the experimental data were not provided in the report Kraxenberger T,2012
Evidence: [TIM]
Reference(s): [1] Fried L., et al., 2013
[2] Kraxenberger T., et al., 2012
TF binding sites (TFBSs)
[PyrR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PyrR-BTS activator yhjXp 3712091 3712101 -52.5 tccgttctgaTGGCATTTCATgccgtttttc nd [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [CV(GEA/SM)], [CV(GEA/SM/GS)], [CV(SM)], [GEA], [GS], [SM] [1], [3]
proximal PyrR-BTS activator yhjXp 3712112 3712122 -73.5 tcctgaactaAGGCATTTCATtccgttctga nd [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/GS)], [CV(GEA/GS)], [CV(GEA/SM)], [CV(GEA/SM/GS)], [CV(SM)], [GEA], [GS], [SM] [1], [3]


Evidence    

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [CV(GEA/GS)] cross validation(GEA/GS)

 [CV(GEA/SM)] cross validation(GEA/SM)

 [CV(GEA/SM/GS)] cross validation(GEA/SM/GS)

 [CV(SM)] cross validation(SM)

 [GEA] Gene expression analysis

 [GS] genomic SELEX

 [SM] Site mutation


Reference(s)    

 [1] Fried L., Behr S., Jung K., 2013, Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli., J Bacteriol 195(4):807-15

 [2] Kraxenberger T., Fried L., Behr S., Jung K., 2012, First insights into the unexplored two-component system YehU/YehT in Escherichia coli., J Bacteriol 194(16):4272-84

 [3] Miyake Y., Inaba T., Watanabe H., Teramoto J., Yamamoto K., Ishihama A., 2019, Regulatory roles of pyruvate-sensing two-component system PyrSR (YpdAB) in Escherichia coli K-12., FEMS Microbiol Lett 366(2)

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