RegulonDB RegulonDB 10.6.3: Operon Form
   

mlrA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mlrA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Gene(s): mlrA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: mlrAp
+1: 2214840
Sigma Factor: Sigma70, Sigma38
Distance from start of the gene: 26
Sequence: tagatgcacaaaaaacgggcaaaactacctggttcgcaaaactgcgtctaaagttaaaccGggacctcgcgagcaagggtg
                          -35               -10             +1                   
Note(s): Regulation of the mlrAp promoter has been described Brown PK,2001. The mlrA gene is RpoS regulated in an avian pathogenic Escherichia coli strain Brown PK,2001.
Evidence: [HIPP]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Pesavento C., et al., 2012
TF binding sites (TFBSs)
[FliZ,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FliZ1 repressor mlrAp 2214816 2214845 -10.0 acctggttcgCAAAACTGCGTCTAAAGTTAAACCGGGACCtcgcgagcaa nd [BPP], [GEA], [SM] [2], [3]
Note(s): 1No DNA consensus sequence that is recognized by FliZ has been established. Therefore, for this DNA-binding site we added the complete region, determined by footprinting analysis Pesavento C,2012 to which FliZ binds.1No DNA consensus sequence that is recognized by FliZ has been established. Therefore, for this DNA-binding site we added the complete region, determined by footprinting analysis Pesavento C,2012 to which FliZ binds.


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Pesavento C., Hengge R., 2012, The global repressor FliZ antagonizes gene expression by σS-containing RNA polymerase due to overlapping DNA binding specificity., Nucleic Acids Res 40(11):4783-93

 [3] Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R., 2008, Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli., Genes Dev 22(17):2434-46

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